1. Figure S4. A 30 32 34 36 38 40 42 44 46 48 50
time (min)
0.000
0.010
0.020
0.030
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ABS (AU) at 520 nm
PAP1 +AOMT
Mv3R
Mv3G
Dp3R a3
PAP1 +AOMT::GFP
Mv3R B
Mv3G
Dp3R a3
Figure S4. Anthocyanin profiles of transient transformed tobacco co-expressing PAP1 and AOMT (A), or co-expressing PAP1 and AOMT::GFP (B). The tobacco leaf sectors (200 mg FW) were excised 96 h after Agrobacterium-mediated transformation. Anthocyanin contents were analysed using HPLC-DAD. Anthocyanins were monitored at 520 nm. The data shown are representative of three independent experiments. Key: Dp3R, delphinidin 3-O-rutinoside; Mv3G, malvidin 3-O-glucoside; Mv3R, malvidin 3-O-rutinoside. Peak a3, malvidin 3-O-rutinoside O-hexoside.