1. Ryan Simpson Adrian Smalley Jenessa Dilley
Hair as a Biological Indicator of Drug Use, Drug Abuse and Chronic Exposure to Environmental Toxins
Ryan Simpson
Adrian Smalley
Jenessa Dilley

2. Advantages Larger detection windows
From 3 days to years
Depending on the length of the hair shaft
Evaluation of long to short term history
Discriminative
Active and passive exposure
Non-invasive sample collection
Broader Applications

3. Anatomy of Hair

4. Hair Growth Stages
Anagen
Catagen
Telogen
Return to Anagen

5. Incorporation Into Hair
Capillary surrounding follicle
Provides Nutrients
Extraneous Substances
Other levels of incorporation
Tissues
Lymph
Intracellular Fluids

6. Detectable Substances
Opiates
Cocaine
Amphetamines
Cannabinoids
Prescription Drugs
Nicotine
Antidepressants
Antipsychotics

7. Detectable Habits
Chronic Alcohol Consumption
Drug Addiction

8. Environmental Toxins Pollution Pesticides Neonatal Exposure
Work Hazards

9. Sample Collection & Preparation
Vertex
Less variability in growth
Less subject to age and sex related influence
Growth phases are more consistent
Washing
Removes external contaminants
Extractions
Alkaline Digestion
Acidic Digestion
Enzymatic Digestion

10. Hair Analysis Analytical Requirements Immunoassays HPLC & CE
Sensitivity
Specificity
Absence of Matrix Effects
Immunoassays
HPLC & CE
GC-MS & GC-MS/MS
Infrared Microscopy

11. Applications Doping Controls Drug Abuse Studies Forensic Toxicology
Drug History
Type and Extent
Forensic Toxicology
Acute Drug Poisoning

12. Applications Evaluate Environmental Exposure to Toxicants
Clinical Chemistry
Compliance of Patience to Doctor’s Prescription
Clinical Toxicology
Prenatal Exposure Assessment
Diagnostic Biomarkers