1. Microtechnologies for biodetection and diagnostics
Peter Lillehoj, Ph.D.
November 27, 2012

2. Introduction to MEMS Micro-electrical-mechanical systems
Started in mid-late 1980’s
Typical component size: 1-100s µm

3. BioMEMS Manipulate, analyze or process biological samples
Improved performance over conventional technologies
S. Sia Lab, Columbia University
L. Lee Lab, UC Berkeley

4. Microfabrication
Etching
Patterning
Molding
PDMS
Si wafer
Deposition

5. Microfluidic systems: cell trapping & lysis
Buffer flow rate
Trapping efficiency %
Buffer flow rate (µL/min)
0 µL/min
10 µL/min
15 µL/min
C. Wu, P. B. Lillehoj, et al., Biotechnology Journal, 2011

6. Microfluidic systems: cell sorting
Sample inlet
Sheath flow
inlet R1 R3 R2
Separation region C1 C2 C3 P1 P2 P3 S1 S2
P. B. Lillehoj, et al., Lab on a Chip, 2010

7. Biosensing I: capturing
Contact pads
Sensors
Alignment mark
Targeting domain
Peptides C
Binding domain
Au sensor

8. Biosensing II: detecting
P. aerugionsa
S. mutans E1 E2 E3 E4 E1 E2 E3 E4
P. B. Lillehoj, et al., Proc. Of 6th IEEE NANOMED 2012

9. Lab-on-a-chip
Inlet 1
Outlet
Mixer
Capillary Pump
Sensor
Inlet 2
Fluids are autonomously driven within channels via capillary pumping
Detection is carried out using a highly-sensitive rapid electrochemical sensor
P. B. Lillehoj, et al., Lab on a Chip, 2010

10. Electrochemical detection: w/ target
current
time
TMB
TMB

11. Electrochemical detection: w/o target
current
time
TMB
TMB

12. BoNT detection results
BoNT/A Specificity
BoNT/A Detection Limit
BoNT/A could be detected at concentrations down to 5 ng/mL with high specificity
The entire detection process only requires 2 loading steps and can be completed within 20 min

13. Cell phone diagnostics
Highly portable
Cell phones contain a visual display, electrical interface and power source
Widely accessible
>75% of the world are mobile subscribers3
3http://

14. Summary & Conclusions
Microtechnologies enables for the development of next-generation systems