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Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo.

Published byCarmelo Marín Giménez Modified over 6 years ago

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Presentation on theme: "Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo."— Presentation transcript:

1 Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo Rodríguez, Constanza Lopez-Albízu, Lucía Irazu, Karenina Scollo, Sergio Sosa-Estani  The Journal of Molecular Diagnostics  Volume 19, Issue 5, Pages (September 2017) DOI: /j.jmoldx Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Analytical verification of the anticipated reportable range of the satellite DNA (SatDNA) and kinetoplastid DNA (kDNA) real-time quantitative PCR (qPCR) methods. The assays were performed with spiked guanidine-EDTA-blood samples that contained 105 to 0.25 parasite equivalents per milliliter of blood (par. eq./mL), tested in triplicate. Assigned values were plotted on the x axis versus measured values (converted to log10) on the y axis using SigmaPlot version 10.0 (SPSS, Chicago, IL). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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