1. Table S1: Chemical analysis and bacterial abundance of ice meltwater from EME-98-03 and DLE-98-12.
Lee et al, Supporting Online Material

2. Table S2. Analysis of bacterial 16S rRNA clones retrieved from DLE-98-12 and EME-98-03 ice samples.
* Numbers in parentheses denote the number of clones assigned to the group (based on sequence identity) followed by the clone representing the group.
† BLAST analysis was performed via the National Center for Biotechnology Information (NCBI) website ( GenBank accession numbers of closest match sequence is provided in parentheses, along with the source when available.
Lee et al, Supporting Online Material

3. Table S3. Net incorporation and respiration of 3H-labeled thymidine, leucine and 14C-labeled glucose by microrganisms in meltwater samples from DLE and EME H activity is expressed as DPM of thymidine or leucine incorporated into nucleic acids or proteins, respectively, per 2 ml sample of ice melt per incubation time (as indicated). 14C activity is expressed as DPM of 14C-labeled glucose that was respired (CO2) or incorporated into cellular biomolecules. Values shown are average net determinations (experimental minus formalin-fixed controls) ± SD (n=3). All incubations were conducted at 4°C in the dark.
* nd- none detected
§ Nutrient spikes correspond to Media T4 (DLE 98-12) and T3 (EME 98-03)
Lee et al, Supporting Online Material

4. Table S4. Phylotypes of Bacteria-specific 16S rDNA amplicons (upper panel) and isolates (lower panel) resuscitated from DLE meltwater cultures. Number in the parentheses is the frequency of the phylotype among the screened clones (n= 128).
* Numbers in parentheses denote the number of similar clones assigned to the group, followed by the clone representing the group.
† BLAST analysis was performed via the NCBI website ( GenBank accession numbers of closest match sequence is provided in parentheses, along with the source when available.
§ DLE011i is red-pigmented, with only 2 bp mismatches to A. roseus CMS90. DLE004i is non-pigmented, and very rare.
Lee et al, Supporting Online Material