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Photosynthesis Energy & Life
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Overview of Photosynthesis
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Autotrophs Plants and some other types of organisms that contain chlorophyll are able to use light energy from the sun to produce food.
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Autotrophs Autotrophs include organisms that make their own food
Autotrophs can use the sun’s energy directly Euglena-protist
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Heterotrophs Heterotrophs are organisms that can NOT make their own food Heterotrophs can NOT directly use the sun’s energy
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Candles release energy as HEAT & LIGHT
Energy Takes Many Forms such as light, heat, electrical, chemical, mechanical Energy can be changed from one form to another Energy can be stored in chemical bonds & then released later Candles release energy as HEAT & LIGHT
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ATP – Cellular Energy Adenosine Triphosphate
Contains two, high-energy phosphate bonds Also contains the nitrogen base adenine & a ribose sugar
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One phosphate bond has been removed
ADP Adenosine Diphosphate ATP releases energy, a free phosphate, & ADP when cells take energy from ATP One phosphate bond has been removed
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Releasing Energy From ATP
Adding A Phosphate Group To ADP stores Energy in ATP Removing A Phosphate Group From ATP Releases Energy & forms ADP Lose Gain
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More on ATP Cells Have Enough ATP To Last For A Few Seconds
ATP must constantly be made ATP Transfers Energy Very Well ATP Is NOT Good At Energy Storage
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Glucose Glucose is a monosaccharide C6H12O6
One Molecule of glucose Stores 90 Times More Chemical Energy Than One Molecule of ATP
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History of Photosynthesis & Plant Pigments
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Photosynthesis Involves the Use Of light Energy to convert Water (H20) and Carbon Dioxide (CO2) into Oxygen (O2) and High Energy Carbohydrates (sugars, e.g. Glucose) & Starches
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The Photosynthesis Equation
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Pigments In addition to water, carbon dioxide, and light energy, photosynthesis requires Pigments Chlorophyll is the primary light-absorbing pigment in autotrophs Chlorophyll is found inside chloroplasts
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Light and Pigments Photon = Light Energy Unit
Energy From The Sun Enters Earth’s Biosphere As Photons Photon = Light Energy Unit Light Contains A Mixture Of Wavelengths Different Wavelengths Have Different Colors
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Light & Pigments Different pigments absorb different wavelengths of light Photons of light “excite” electrons in the plant’s pigments Excited electrons carry the absorbed energy Excited electrons move to HIGHER energy levels
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Magnesium atom at the center of chlorophyll
There are 2 main types of chlorophyll molecules: Chlorophyll a Chlorophyll b Magnesium atom at the center of chlorophyll
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Chlorophyll a Found in all plants, algae, & cyanobacteria
Makes photosynthesis possible Participates directly in the Light Reactions Can accept energy from chlorophyll b
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Chlorophyll b Chlorophyll b is an accessory pigment
Chlorophyll b acts indirectly in photosynthesis by transferring the light it absorbs to chlorophyll a Like chlorophyll a, it absorbs red & blue light and REFLECTS GREEN
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The Biochemical Reactions
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It Begins with Sunlight!
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Inside A Chloroplast
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Structure of the Chloroplast
Double membrane organelle Outer membrane smooth Inner membrane forms stacks of connected sacs called thylakoids Thylakoid stack is called the granum (grana-plural) Gel-like material around grana called stroma
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Thylakoid membranes Light Dependent reactions occur here
Photosystems are made up of clusters of chlorophyll molecules Photosystems are embedded in the thylakoid membranes The two photosystems are: Photosystem I Photosystem II
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Function of the Stroma Light Independent reactions occur here
ATP used to make carbohydrates like glucose Location of the Calvin Cycle
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Photosynthesis Overview
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Energy Carriers NADP+ = Reduced Form
Nicotinamide Adenine Dinucleotide Phosphate (NADP+) NADP+ = Reduced Form Picks Up 2 high-energy electrons and H+ from the Light Reaction to form NADPH NADPH carries energy to be passed on to another molecule
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Light Dependent Reactions
Occurs across the thylakoid membranes Uses light energy Produce Oxygen from water Convert ADP to ATP Also convert NADP+ into the energy carrier NADPH
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Light Dependent Reaction
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Light Dependent Reaction
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Light Dependent Reaction
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Photosynthesis Begins
Photosystem II absorbs light energy Electrons are energized and passed to the Electron Transport Chain Lost electrons are replaced from the splitting of water into 2H+, free electrons, and Oxygen 2H+ pumped across thylakoid membrane
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Photosystem I High-energy electrons are moved to Photosystem I through the Electron Transport Chain Energy is used to transport H+ from stroma to inner thylakoid membrane NADP+ converted to NADPH when it picks up 2 electrons & H+
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Phosphorylation Enzyme in thylakoid membrane called ATP Synthase
As H+ ions passed through thylakoid membrane, enzyme binds them to ADP Forms ATP
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Light Reaction Summary
Reactants: H2O Light Energy Energy Products: ATP NADPH Molecular Product: O2
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Light Independent Reaction
ATP & NADPH from light reactions used as energy Atmospheric C02 is used to make sugars like glucose and fructose Six-carbon Sugars made during the Calvin Cycle Occurs in the stroma
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Melvin Calvin 1948 Does NOT require sunlight
First to trace the path that carbon (CO2) takes in forming Glucose Does NOT require sunlight Called the Calvin Cycle or Light Independent Reaction Also known as the Dark Reaction
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The Calvin Cycle
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The Calvin Cycle Two turns of the Calvin Cycle are required to make one molecule of glucose 3-CO2 molecules enter the cycle to form several intermediate compounds (PGA) A 3-carbon molecule called Ribulose Biphosphate (RuBP) is used to regenerate the Calvin cycle
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Factors Affecting the Rate of Photosynthesis
Amount of available water Temperature Amount of available light energy
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Increasing levels of CO2 will also cause the rate of photosynthesis to increase then level off.
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Light independent Reaction Summary
Reactants: CO2 Energy Products: ADP + P NADP+ Molecular Product: C6H12O6 49
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