1. Heredity & the Environment
Tobacco Albino Seeds

2. I. Background Information:
An organism’s phenotype or physical appearance for a particular trait is the result of both its genetic makeup and the environment.
Genes may be turned off by environmental factors: light, temperature, etc.
Why use a gene/protein when you don’t need it?
The energy efficiency provided is a survival adaptation

3. I. Background Information:
Tobacco seeds will germinate and grow in as little as three days if they are kept moist.
The seeds contain an embryonic plant along with cotyledons that serve as a food source for the embryonic plant before its leaves develop, produce chlorophyll, and carry out photosynthesis.
Tobacco seedlings that possess a dominant allele for chlorophyll production will produce chlorophyll if environmental conditions are favorable.
However, tobacco seedlings that possess two recessive alleles are genetically programmed to be albino, and will not possess chlorophyll. Therefore, photosynthesis cannot occur in albino plants.

4. II. Research Question:
What about the environment might affect a plants gene expression?
We are testing to see if we get this 3:1 ratio!

5. IV. Model
What we did in class

6. V. Experimental Design
Research Questions (2): Null Hypothesis: Alternative Hypothesis 1 (light): Alternative Hypothesis 2 (chosen variable): Independent Variable: Experimental Group(s): LIST ALL. Control Group: Normal light and distilled water Dependent Variable: % Germination Constants:

7. VI. Materials & Procedures
Water, tap
Marker, masking tape
3 Disposable plastic petri dishes (100 x 15 mm) – 2 were done by Mr. Koons as a class set
Pipet
Paper towel
Tobacco seeds 30/experimental group
Lightproof storage area or covers (from parents heterozygous for albinism)
Different colored lights, salt solution, OR vinegar solution

8. Procedure (paste into your notebook):
With a piece of tape: label the bottom of one Petri dish “light”; the bottom of another Petri dish “dark. Label all with your group name and date.
Place a piece of moistened filter paper into the bottom half of each Petri dish.
Sprinkle 30 tobacco seeds evenly over the moistened paper in each Petri dish. Use a toothpick to spread out in the two dishes.
Replace the cover on each Petri dish. Place the dish labeled “light” in a well- lighted area under the grow lights. Place the dish labeled “dark” in a completely darkened area where it will receive absolutely no light. (place your Petri dish in the designated drawer for your class). Place your third petri dish in the area or environmental condition that your group has selected.
Allow the seeds to germinate for one week. Let the dishes sit undisturbed except to add water if the filter paper should become dry. If the dish in the dark needs added water, add it quickly and in a reduced light environment. (this is your groups responsibility – if your seeds dry out you will receive 0 for the results)
Observe the 3 Petri dishes at the end of one week. Some seedlings will be pale in color (albino) with little or no green pigment. Other seedlings will have green areas forming. When nearly all the seedlings have germinated, count each seedling as either green or albino. Record your results in the data table below:

9. ADD # SEEDLINGS AND % TO EACH DATA TABLE – MAKE SURE ALL 3 ARE IN YOUR NOTEBOOK16 80 4 20

10. GRAPH THE PERCENT FOR EACH COLOR FOR EACH VARIABLE (LIGHT, DARK AND ___________) Add a GRAPH TO YOUR NOTEBOOK – YOU WILL ALSO NEED ONE FOR THE FORMAL LAB REPORT THAT CAN BE CREATED IN EXCEL, GRAPHPAD OR WORD

11. WHEN DO I PERFORM A CHI SQUARE:

12. PERFORM A CHI SQUARE FOR SEEDLINGS IN THE LIGHT, IN THE DARK AND THE THIRD VARIABLE – THE EXPECTED % FOR EACH SHOULD BE 75% GREEN AND 25% WHITE
Do this one for practice; we should get a low chi square value! 80 75 5 25
0.33 20 25 5 25 1
O.33+1 = 1.33

13. DO 3 SEPARATE CHI SQUARES – ONE FOR NORMAL LIGHT (for practice), ONE FOR SEEDS IN DARK AND ONE FOR SEEDLINGS EXPOSED TO THE VARIABLE FOR OUR GROUP

14. ALWAYS COMPARING TO THE NULL HYPOTHESIS:

16. WHAT DO I DO WITH THE CHI SQUARE VALUE: