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Role of the basophil activation test in the diagnosis of local allergic rhinitis  Enrique Gómez, PhD, Paloma Campo, MD, PhD, Carmen Rondón, MD, PhD, Esther.

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Presentation on theme: "Role of the basophil activation test in the diagnosis of local allergic rhinitis  Enrique Gómez, PhD, Paloma Campo, MD, PhD, Carmen Rondón, MD, PhD, Esther."— Presentation transcript:

1 Role of the basophil activation test in the diagnosis of local allergic rhinitis 
Enrique Gómez, PhD, Paloma Campo, MD, PhD, Carmen Rondón, MD, PhD, Esther Barrionuevo, MD, Natalia Blanca-López, MD, PhD, María José Torres, MD, PhD, Rocío Herrera, RN, Luisa Galindo, RN, Cristobalina Mayorga, PhD, Miguel Blanca, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 132, Issue 4, Pages e5 (October 2013) DOI: /j.jaci Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 BAT after adding D pteronyssinus at 5, 20, and 50 ng/mL. The horizontal line indicates the cutoff point of the assay. Der p, D pteronyssinus; IgE C+, positive IgE control; SI, stimulation index. A, Patients with AR (n = 13). B, Patients with LAR (n = 16). C, Healthy control subjects (n = 14). D, Patients with NAR (n = 10). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig E1 BATs performed in 4 patients with LAR by using both the standard assay (gray bars) and wortmannin pretreatment (full bars). Results are plotted side by side for each dilution. The horizontal line indicates the cutoff point of the assay. DP, D pteronyssinus; fMLP, N-formyl-methionine-leucine-phenylalanine; SI, stimulation index. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E2 BAT dose-response curves with serial dilutions of D pteronyssinus (DP) extract (1-400 ng/mL) in 6 patients with AR and 6 control subjects. Results are expressed as the stimulation index (SI). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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