1. The Microscope

2. The Microscope
Enables you to see things that are too small to be seen with the unaided eye.
Allows you to look more closely at the finer details of things.

3. What the microscope sees
What you see
What the
microscope sees
Blood

4. What the microscope sees
What you see
What the
microscope sees
Fly

5. What the microscope sees
What you see
What the
microscope sees
Leaf

6. What the microscope sees
What you see
What the
microscope sees
Eyelash

7. What the microscope sees
What you see
What the
microscope sees
Strawberry

8. Microscope Parts Eyepiece (10X) Coarse Adjustment Body Tube
Fine Adjustment
Nosepiece
Arm
Scanning Objective (4X)
High Power Objective (40X)
Stage
Stage Clips
Base
Diaphragm
Light Source

9. Parts at Work Eyepiece – What you look through. Objectives
by itself enlarges the specimen by 10X
Objectives
Scanning Power (4X) = to locate and focus the specimen, allows you to see the largest amount of a specimen. (Red)
Low Power (10X) = to examine details (Yellow)
High Power (40X) = to examine the finest details (Blue)

10. Parts at Work
Coarse Adjustment – to focus in the specimen under scanning and low power.
Fine Adjustment – to focus in the specimen under high power ONLY.
Arm & Base – used to carry the microscope.
Nosepiece – holds the objective lenses.

11. Parts at Work
Stage & Stage Clips – holds the slide with specimen securely.
Diaphragm – adjusts the amount of light shining through specimen.
Light Source – provides light that shines through the specimen.

12. Magnifications Part Magnification Eyepiece Scanning Objective
Low Power Objective
High Power Objective
10X 4X
10X
40X

13. Total Magnification
The total amount a specimen is magnified due to the combined magnifications of the eyepiece and objective lenses being used.
Eyepiece
Objective
Total Magnification X =
10X 5X
50X
15X
15X
225X
10X
10X
100X
20X
40X
800X

14. Total Magnifications Which total magnification would allow you to see:
Eyepiece
Objective
Total Magnification 10 4 40
100
400
Which total magnification would allow you to see:
The largest amount of the total specimen?
The finest details of the specimen?
Which set of lenses would you use to find and focus a specimen?
40X
400X
40X

15. Total Magnification B. A. C. _____ 1. Scanning Power (40X)
Used to find a specimen
_____ 2. Low Power (100X)
To view more detail of the specimen.
_____ 3. High Power (400X)
To view the greatest detail of the specimen. A. B. C. C.

16. Field of View
The diameter of the circle of light that you see when looking through the eyepiece is called the Field of View.
What is the field of view as
seen in the image?
_______ cm or _______ mm
1.5 15

17. Field of View
As the power of the microscope becomes greater the field of view gets smaller.
When measuring field of view always read it from the diameter.
Scanning Power: (40X)
High Power: (400X)
4.0 mm
.5 mm

18. Field of View
If every = 1mm, what would be the diameter of this field of view?
If every = 1mm, what would be the approximate size of letter “e”? A.
6 mm B.
2 mm