1. Volume 41, Issue 1, Pages 49-56 (January 2010)
Use of the Induced Membrane Technique for Bone Tissue Engineering Purposes: Animal Studies 
Véronique Viateau, DMV, PhD, Morad Bensidhoum, PhD, Geneviève Guillemin, PhD, Hervé Petite, PhD, Didier Hannouche, DM, PhD, Fani Anagnostou, DM, Philippe Pélissier, DM 
Orthopedic Clinics 
Volume 41, Issue 1, Pages (January 2010)
DOI: /j.ocl
Copyright © Terms and Conditions

2. Fig. 1
Immunohistochemical staining of the encapsulation membrane sampled at time of cement explantation from a sheep metatarsal defect (A). Type I collagen staining shows that the membrane is mainly composed of an alveolar organization of parallel bands of collagen fibers (B).
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions

3. Fig. 2
Immunohistochemical staining of the encapsulation membrane sampled at time of cement explantation from a sheep metatarsal defect. Lycopersicon esculentum lectin labeling of vessels in longitudinal (A) and transverse (B) sections demonstrating the perpendicular orientation of the membrane vascularization (arrows).
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions

4. Fig. 3
Representative radiogram (A) and 3-dimensional tomodensitometry reconstruction (B) of a 25-mm-long metatarsal critical size defect of a sheep implanted with fragmented autologous bone, 6 months after cement removal. Microradiography (frame C), and histology (D) of a representative 2-dimensional section were performed at this time point. The stain used for the histology was van Gieson picro-fushine.
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions

5. Fig. 4
Immunohistochemical staining (B) of a decalcified section (A) of a massive construct seeded with autologous GFP-transduced MSCs 2 months after implantation in a subcutaneous pouch delineated by a PMMA-induced membrane in sheep. Bone formation (white arrow) and osteoblasts expressing eGFP (black arrow) are observed. The stain used for the histology (A) was hematoxylin-eosin-saffron (HES).
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions

6. Fig. 5
Representative radiogram (A) and 3-dimensional tomodensitometry reconstruction (B), of a 25-mm-long metatarsal critical size defect of a sheep implanted with MSCs/coral constructs, 6 months after cement removal. Microradiography (C), and histology (D) of a representative 2-dimensional section were performed at that time point. The histologic stain used was von Gieson picro-fushine.
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions

7. Fig. 6
Quantification of bone formation. *P<.05 compared with defects left empty. **P<.05 compared with defect filled with coral alone.
Orthopedic Clinics  , 49-56DOI: ( /j.ocl )
Copyright © Terms and Conditions