1. Acute myeloid leukemia creates an arginase-dependent immunosuppressive microenvironment
by Francis Mussai, Carmela De Santo, Issa Abu-Dayyeh, Sarah Booth, Lynn Quek, Rosanna M. McEwen-Smith, Amrana Qureshi, Francesco Dazzi, Paresh Vyas, and Vincenzo Cerundolo
Blood
Volume 122(5):
August 1, 2013
©2013 by American Society of Hematology

2. Arginine metabolism regulates the suppressive activity of AML blasts.
Arginine metabolism regulates the suppressive activity of AML blasts. (A) AML blasts from 15 patients were cultured with allogeneic T cells in a MLR. The ratio of AML cells:T cells ranged from 1:1 to 1:0. T-cell proliferation was measured by 3H-thymidine incorporation after 4 days. AML blasts from 12/15 patients strongly suppressed T-cell proliferation. Patients are identified by unique symbols, which are used consistently throughout the manuscript. (B) Expression of arginase II in blasts from patients with AML was confirmed by confocal microscopy. (Left) Staining with DAPI (nuclear stain) and secondary antibody alone; (right) staining with DAPI, anti-human arginase II antibody, and secondary antibody. Scale bar = 10 μm. (C) AML blasts release arginase II into the microenvironment. Supernatants from cultures (24 hours) of patients’ AML blasts were analyzed by ELISA for arginase II and arginase I (***P = .0001). (D) AML blasts from patients were cultured with allogeneic T cells in a MLR in the presence of the enzyme-specific inhibitors for arginase (NOHA) and iNOS (L-NMMA). The ratio of AML cells:T cells ranged from 1:1 to 1:8. T-cell proliferation was measured by 3H-thymidine incorporation after 4 days. Culture with the enzyme inhibitors restores T-cell proliferation. Data are representative of 5 independent experiments (error bars, SD).
Francis Mussai et al. Blood 2013;122:
©2013 by American Society of Hematology

3. AML blasts extend their suppressive microenvironment through high concentrations of active arginase II in patient plasma.
AML blasts extend their suppressive microenvironment through high concentrations of active arginase II in patient plasma. (A) Arginase activity from plasma of 15 patients with AML and 15 healthy donors was analyzed (***P = .0001). Fifty microliters of patient plasma was tested for the ability to convert arginine into urea, using a colorimetric assay. (B) Plasma (50 μL) from 17 patients with AML and 21 healthy donors was analyzed for arginase II concentration by ELISA (***P = .0001). (C) T-cell proliferation of alloreactive T cells stimulated by allogeneic DC in a total volume of 200 μL, with 50 μL of plasma from patients with AML collected at time of diagnosis or from healthy donors (***P = .0001). (D) T cells from healthy donors were cultured in a MLR in the presence of plasma from patients with AML and the enzyme-specific inhibitors for arginase (NOHA) and iNOS (L-NMMA) or arginine. T-cell proliferation was measured by 3H-thymidine incorporation after 4 days. Culture with the enzyme inhibitors or arginine replacement restores T-cell proliferation. Data are representative of 5 independent experiments (error bars, SD).
Francis Mussai et al. Blood 2013;122:
©2013 by American Society of Hematology

4. AML blasts increase CD206 expression on monocytes.
AML blasts increase CD206 expression on monocytes. (A) Transwell culture of CFSE-labeled monocytes from healthy donors with AML blasts from patients upregulates CD206 expression on monocytes. Monocytes from healthy donors were placed in the lower well and AML blasts from patients were placed in the upper well of a transwell assay system. Representative flow cytometry plots from 1 patient of 11 tested are shown. (B) Coculture of CFSE-labeled monocytes from healthy patients with supernatants (50% of final volume) from AML blasts upregulates CD206 on monocytes, as analyzed by flow cytometry. (C) Upregulation of CD206 on monocytes cultured with plasma of 15 patients with AML or 15 healthy donors (P < .0001). Percentage of CD206+ cells is shown. (D) Upregulation of CD206 on monocytes cultured with plasma of patients with AML with or without arginine (100 ng/mL) and inhibitors (0.5 mM). Data are representative of 3 independent experiments (E) Staining of bone marrow from patients with AML at diagnosis with anti-human CD206 (center), with DAPI alone (left), and with anti-arginase II (right). Representative marrow from a single patient with AML shown of 6 (samples obtained from University of Oxford Biobank).
Francis Mussai et al. Blood 2013;122:
©2013 by American Society of Hematology

5. Monocytes from NOD-SCID mice engrafted with human AML express increased YM-1.
Monocytes from NOD-SCID mice engrafted with human AML express increased YM-1. (A) NOD-SCID mice were injected with AML cells from patients. After engraftment, bone marrow was harvested from the femurs, and the expression of YM-1 on murine monocytes (Ly6C+) was assessed by flow cytometry. (B) Increased percentage of YM-1 positive monocytes in NOD-SCID mice engrafted with human AML. NOD-SCID mice were injected with AML cells from patients. After engraftment, bone marrow was harvested from the femurs, and the percentage of YM-1+ Ly6C+ murine monocytes was assessed by flow cytometry (***P = .0001).
Francis Mussai et al. Blood 2013;122:
©2013 by American Society of Hematology

6. The AML microenvironment suppresses GMP and CD34+ progenitors.
The AML microenvironment suppresses GMP and CD34+ progenitors. (A) plasma from patients with AML suppresses murine GMP proliferation. CFSE-labeled murine GMPs were isolated and cultured in the presence of plasma from patients with AML and in the presence of L-NMMA (0.5 mM) and NOHA (0.5 mM). A representative histogram plot of 5 patient AML plasma experiments is shown. Independent experiments were performed on 2 separate occasions. (B) AML-induced GMP suppression is overcome by the addition of L-NMMA and NOHA. GMPs cultured in the presence of AML plasma (50% of final volume) have significantly reduced proliferation compared with those cultured in the presence of healthy donor plasma (P = .0001) or AML plasma with L-NMMA (0.5 mM) and NOHA (0.5 mM) (P = .0001). (C) Plasma from patients with AML suppresses human CD34+ HSC proliferation. CFSE-labeled human CD34+ HSCs were isolated and cultured in the presence of plasma from AML patients and in the presence of L-NMMA (0.5 mM) and NOHA (0.5 mM). Data are representative of 5 experiments with plasma from patients with AML. Independent experiments were performed on 2 separate occasions. (D) AML-induced human CD34+ HSC suppression is overcome by the addition of L-NMMA and NOHA. CD34+ HSCs cultured in the presence of AML plasma have significantly reduced proliferation compared with those cultured in the presence of healthy donor plasma (P = .0002) or AML plasma with L-NMMA and NOHA (P = .003). Independent experiments were performed on 2 separate occasions.
Francis Mussai et al. Blood 2013;122:
©2013 by American Society of Hematology