1. Extension Attachment Contraction Release Recycling C o n t r a c i x eF
Attachment
Contraction
Release
Recycling

2. Attachment and Contraction

3. FN Bead Binding Movement and Release
Displacement

4. How does a cell treat the ECM?

5. Unstressed Attachments
Choquet et al., Cell. 88:39 (1997)

6. Force Causes Reinforcement

7. Force Causes GFP-Paxillin Accumulation in RPTPa+/+ with FN
Without Force
FN Bead
Con A Bead

8. RPTPa Deletion Blocks But Transfection Restores Binding
-/- Cells with Force
-/- Transfected wtRPTPa

9. Substrate Rigidity Can Control Direction of Motility
Non-Rigid
Rigid

13. Horwitz, Scientific American

14. Summary of Course Lectures
1-Intro 2-Diffusion 3-Diff.Transport 4-Nuclear Organization 5.Nuc.Binding 6 DNA Replication 7 RNA Transcription 8-RNA FactorBind.doc 9-Fluid Bilayer 10-Guoy-Chapman 11-Membrane Potential 12 Protein Translation
13-Cytoskeleton
14-Self-Assembly
15-16Molec.Mech.Q.doc
16-Molecular Motors
17-Mechanics
18-Motor Kinetics
19 Protein Translocation
20 Fission and Fusion
21 Proteol-Degrad
22 Signal Integration
23 Cell Migration
24 Cell Motility ()