1. The Immune System Second Edition
Peter Parham
The Immune System Second Edition
Chapter 2
Antibody Structure and the Generation
of B-Cell Diversity
Copyright © 2005 by Garland Science Publishing

2. Figure 2-1

3. IgG
Figure 2-2

4. Figure 2-3

5. Figure 2-4 pentamers Monomers/dimers
Immunlglobulin Isotypes or Classes

6. Figure 2-5

7. Figure 2-6

8. Figure 2-7 part 1 of 2 110 AA light-chain V domain
HV domains- hypervariable
FR domains- framework

9. Figure 2-7 part 2 of 2

10. Figure 2-8
Poliovirus
VP1- blue, contains several epitopes (white) that can be recognized by humans

11. Figure 2-9

12. Pockets Grooves Extended surfaces
Figure 2-10

13. Figure 2-11

14. Figure 2-12 part 1 of 3
Monoclonal Antibody

15. Figure 2-12 part 2 of 3

16. Figure 2-12 part 3 of 3

17. Figure 2-13
Flow cytometer

18. Gene rearrangements during B-cell development
V-variable, J-joining, D-diversity gene segments
l - 30 V & 4 pairs J & C (light chain) chs22
k – 40 V & 5 J & 1 C (50% have 2x V) (light chain) chs2
H – 65 V & 27 D & 6 J chs L-leader sequences
Figure 2-14

19. J-joining
Figure 2-15 part 1 of 2

20. D-diversity
Figure 2-15 part 2 of 2

21. Figure 2-16

22. Figure 2-21 part 1 of 2

23. Figure 2-21 part 2 of 2

24. Figure 2-23
Disulfide-linked
Transmembrane proteins - invariant

25. Figure 2-24 part 1 of 2

26. Figure 2-24 part 2 of 2

27. Figure 2-25 part 1 of 2 4 CDRs now altered, limited variation Ab1 Ab2
AA change

28. Figure 2-25 part 2 of 2
All 6 CDRs now altered

29. Figure 2-26 part 1 of 2

30. Figure 2-26 part 2 of 2

31. Figure 2-27

32. Figure 2-28

33. Figure 2-29 part 1 of 2

34. Figure 2-29 part 2 of 2

35. At mucosal surfaces
Figure 2-30

36. Summary Biosynthesis of IgM in B cells

37. Figure 2-31 part 2 of 2

38. Figure 2-32