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Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R. K

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Presentation on theme: "Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R. K"— Presentation transcript:

1 Bacterial diversity in aortic aneurysms determined by 16S ribosomal RNA gene analysis 
Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R.K. Eribe, PhD, Jørn A. Aas, DDS, PhD, Per S. Lingaas, MD, Odd Geiran, MD, PhD, Leif Tronstad, DMD, PhD, Ingar Olsen, DDS, PhD  Journal of Vascular Surgery  Volume 44, Issue 5, Pages (November 2006) DOI: /j.jvs Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Polymerase chain reaction products from aortic aneurysm samples using eubacterial primers to amplify approximately 500 bp of the 16S rRNA gene. Lane M, Molecular weight marker (1Kb DNA Ladder, Invitrogen). Lanes 1 to 10, DNA extracted from patients’ aneurysm samples. Lanes N, negative controls (sterile water). Lane P, positive control (purified DNA from E. coli). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Phylogenetic tree of representative 16S rDNA sequences obtained from 10 aortic aneurysm samples. The sequences were aligned using ClustalV, and the unrooted tree was constructed using the DNASTAR program, showing two major clusters at 10% nucleotide sequence difference. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

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