1. A Systematic Approach to Novel Virus Discovery in Emerging Infectious Disease Outbreaks 
Siddharth Sridhar, Kelvin K.W. To, Jasper F.W. Chan, Susanna K.P. Lau, Patrick C.Y. Woo, Kwok-Yung Yuen 
The Journal of Molecular Diagnostics 
Volume 17, Issue 3, Pages (May 2015)
DOI: /j.jmoldx
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1 Algorithm for novel virus discovery.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Methods of virus detection applied to novel coronavirus discovery. A: Vero kidney epithelial cells infected by Middle East respiratory syndrome–coronavirus (MERS-CoV) showing cytopathic effect in the form of cell shrinkage and syncytium formation. B: Electron micrograph of severe acute respiratory syndrome coronavirus (SARS-CoV) showing characteristic enveloped virus particles with protruding spikes. C: Gel electrophoresis image of DNA amplified by coronavirus pol gene-derived consensus primer RT-PCR. Lane 1, bacteriophage ΦX174 DNA HaeIII digest marker; 2, negative sample; 3, negative control; 4, positive control; 5, nasopharyngeal aspirate sample with human coronavirus HKU1. D: Phylogenetic analysis of selected human and animal coronaviruses on the basis of the pol gene by the neighbor joining method. Lineage A betacoronavirus (A), lineage B betacoronavirus (B), and lineage C betacoronavirus (C).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions