1. WSSP-14 Chapter 6
Editing the DNA sequence waveforms
atttaccgtg ttggattgaa attatcttgc atgagccagc tgatgagtat gatacagttt tccgtattaa taacgaacgg ccggaaatag gatcccgatc atgattgctt caatattttc acttcaatga ttggttctaa gcattcgaat gcgtacccgt ttgattaata tttccatttc tgtcccagtt tttaattttc atttcttttg gttaaaaaat tcccagtctc ttgaatgctt ttctaaaatc tttaattcaa ttatttatta gaatcttctg ttttgagaac tttgtaatgt aattaaataa tttgatgaaa tgattatgaa tgcgaataaa ttattaattt accgtgctga ttggattgaa attatcttgc atgagccagc tgatgagtat gatacagttt tccgtattaa taacgaacgg ccggaaatag gatcccgatc atgattgctt caatattttc acttcaatga ttggttctaa gcattcgaat gcgtacccgt ttgattaata tttccatttc tgtcccagtt tttaattttc atttcttttg gttaaaaaat tcccagtctc ttgaatgctt ttctaaaatc tttaattcaa ttatttatta gaatcttctg ttttgagaac tttgtaatgt aattaaataa tttgatgaaa tgattatgaa tgcgaataaa ttattaattt accgtgttgg attgaaggta attatcttgc atgagccagc tgatgagtat gatacagttt

2. DSAP: Edit Sequence Page
© 2014 WSSP

3. p. 6-1
© 2014 WSSP

4. Edit options
in 4 Peaks
Edit options
in Finch TV
p. 6-2
© 2014 WSSP

5. A dye blob at the beginning of the For sequence
Before Editing
After Editing
p. 6-2
© 2014 WSSP

6. Examples of sequences with background noise
© 2014 WSSP

7. Sequence that is not reliable
p. 6-3
© 2013 WSSP

8. An edit of an N in the EX1.14 For sequence
p. 6-4
© 2014 WSSP

9. Edits of bad spaced miscalled bases in the EX1.14 sequence
© 2014 WSSP

10. Paste edited sequence into the DSAP dialog box
© 2014 WSSP

11. What should the N be? A C G T
Can not tell

12. Is this sequence properly called ? Yes No Can not tell
© 2014 WSSP

13. How should this sequence be handled?
Keep the C and keep on processing it.
Delete the C and keep on processing it.
Crop the 3’ end at the base before and keep on processing it.
Indicate that the sequence is unreadable, and can not submit.
© 2014 WSSP

14. What should the N be? A C G T
No base

15. What should the GG be? GG G CG
Can not tell

16. What should the AA be? AA A TA
Can not tell

17. Is this sequence properly called ?
Yes No
Can not tell
© 2014 WSSP

18. How should this sequence be handled?
Keep the T and keep on processing it.
Change to an A and keep on processing it.
Crop the 3’end at the base before and keep on processing it.
Indicate that the sequence is unreadable, and can not submit.
After cropping the sequence will be too short to submit
© 2014 WSSP