1. Homework: Homework Packet 5
Warm up: What is the function of DNA? Flush: Do you prefer dogs or cats? Why?
Homework: Homework Packet 5

2. Agenda Transcription ‘Expert Groups’ Transcription notes
Diagram of transcription
Exit Ticket

3. Transcription Explore
You will read and annotate the text focusing on only ONE topic for your group
Topics to Assign
1. RNA vs. DNA Structure
2. Definition and Location of Transcription
3. Examples of mutations that occur
4. Importance of Transcription * (leave this out for groups of 3)

4. Protein Synthesis Overview
Making proteins!
Two steps
Transcription
Translation
Today we will focus on transcription
Transcription is taking DNA and making mRNA

5. Transcription Taking a piece of DNA and making messenger RNA (mRNA)
The first step of protein synthesis
How DNA turns its code into a protein

6. Location of Transcription
In eukaryotes: transcription occurs in the nucleus
Nucleus protects DNA
mRNA acts as messenger that leaves the nucleus so DNA can stay safe
In prokaryotes: occurs in the cytosol since prokaryotic cells do NOT have a nucleus

7. DNA vs. RNA Structure DNA: RNA: Double stranded
Contains adenine, thymine, guanine, and cytosine
Has a deoxyribose sugar
RNA:
Single stranded
Contains adenine, uracil, guanine, and cytosine
Has a ribose sugar

8. Base Pairing in Transcription
Base pairing is also important in transcription
Base pairing allows a molecule of mRNA To be made from DNA
Similar rules
G-C
A-U
T-A
RNA polymerase is the enzyme that helps form DNA-RNA base pairs

9. Transcription
RNA Polymerase is an enzyme (so a protein) that plays down new RNA nucleotides to create the mRNA strand

10. DNA Mutations Point Mutation (Substitution)
One base pair is substituted for another in the DNA Sequence
Frameshift Mutation (insertion/deletion)
Base pairs are added or deleted to the DNA sequence
*If the DNA sequence changes, the RNA sequence will also change!

11. Transcription and Mutations Practice

12. Transcription Diagrams

13. Transcription Diagram
Using your notes, draw an illustration or picture of transcription in a eukaryotic and prokaryotic cell. Include:
Both types of cells (one with nucleus, one without)
Structure of DNA vs. RNA
Base pairing occurring
Function of RNA polymerase
Show an example of one mutation
Color and be creative. This will help you remember the details in your notes.
To get FULL CREDIT you must show a picture and give a written explanation for the 5 items above

14. Exit Ticket
1. Compare and contrast the location of transcription in prokaryotes and eukaryotes
2. Draw a picture that shows the difference between the structure of DNA and RNA
3. Create a DNA strand and show the RNA strand that would be made during transcription
4. Explain one example of a mutation that can occur