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Quantification of intracellular fuzzy staining (A) and of spherical bodies (B) in root cells upon an NaCl treatment. Quantification of intracellular fuzzy staining (A) and of spherical bodies (B) in root cells upon an NaCl treatment. The figure represents the proportion of root epidermal cells 1 cm far from the apex that show intracellular fuzzy staining (A) and spherical bodies (B) in control conditions (white) and after treatment with 100 mm NaCl during 2 h (gray) or 4 h (black). Data were obtained from transgenic plants that express the following constructs: GFP-PIP2;1 (two independent transgenic lines; n ≥ 7 plants per treatment), GFP-PIP2;1-S280A (three independent transgenic lines; n = 9 per treatment), GFP-PIP2;1-S283A (two independent transgenic lines; n ≥ 7 per treatment), and GFP-PIP2;1-S283D (two independent transgenic lines; n ≥ 6, per treatment). The numbers on the graph correspond to the total number of observed cells and are identical in the two figures. The error bars represent S.D. Sodana Prak et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology
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