1. صدق الله العلي العظيم (سورة النساء:113)
بسم الله الرحمن الرحيم
وَأَنزَلَ اللَّهُ عَلَيْكَ الْكِتَابَ وَالْحِكْمَةَ وَعَلَّمَكَ مَا لَمْ تَكُنْ تَعْلَمُ وَكَانَ فَضْلُ اللَّهِ عَلَيْكَ عَظِيمًا
صدق الله العظيم
صدق الله العلي العظيم
(سورة النساء:113)

2. Determination and enzymatic removal of Some Pollutants in Effluent of Water Treatment Strains for Petroleum Refinery Project

3. Al-Dora Oil Refinery
Al- Dora oil refinery is one of the major refineries in Iraq. This refinery operates at 24 hours per day, very large amounts of crude oil are refined (5x106 M3/year) to produce different oil cuts such as benzene, gasoil, asphalt,…. etc.

4. Crude oil is converted into higher-value fuel products
Crude oil is converted into higher-value fuel products. First, the raw crude oil is washed in a de-salter and heated. Next, it enters by the vacuum fractionator, followed by the atmospheric crude fractionator

6. General Composition of Various Hydrocarbon Products

7. Wastewater Treatment
Oily water treatment is a one of the most popular engineering and industrial services in the field of industrial water treatment. Human being activities are sending everyday 5.2 x 105 to 2 million gallons of crude oil or its product to the sea. This encourages the engineering industry to develop various techniques and produce more research to deal with this urgency problem

9. Phenols Effect of Human Health
Phenol is a white hygroscopic crystalline solid at room temperature with an acrid smell. The chemical formula of phenol is C6H6OH, and its molecular mass is 94.11
Phenols and phenolic compounds are of environmental concern due to their toxicity and being as ubiquitous contaminants in the environment

10. Benzidines Effect of Human Health
Benzidine is a moderately persistent pollutant in the environment and the exposure to populations with benzidine’s waste-dis-posal is a matter of great concern with regard to human health
The compounds are highly toxic, carcinogenic, mutagenic and teratagenic, stable in water, almost non-biodegradable and irremovable with conventional water treatment technology, thus internationally recognized as priority pollutants

11. Determination of Priority Pollutant Phenols (PPP) in Water

13. Determination of Priority Pollutant Benzidine

15. Enzymes in Wastewater Treatment
A peroxidase-based treatment method to remove aromatic compounds from wastewater employed the peroxidase from Soybean Peroxidase (SBP) or horseradish (HRP), in the presence of hydrogen peroxide, catalyzing the oxidation of over 30 different phenols and aromatic amines.

16. Soybean Peroxidase (SBP)
Soybean peroxidase (SBP) is a hemoprotein oxidoreductase-class enzyme extracted from the soybean seed coat (hull).. The seed hull contains a mixture of soluble proteins of different structural characteristics (size and charge).

17. Structure of Soybean Peroxidase

18. Peroxidase Mechanism

19. High-Performance Liquid Chromatography HPLC

20. Solid Phase Extraction (SPE)
one of the most substantial steps in analytical chemistry, particularly when the samples need to accomplish a accurate analysis of sophisticated sample mixtures Composed of many components.

21. List of PPB compounds and their chemical structures and abbreviations

22. Purification and Separation of Priority Pollutant Benzidines (PPB) by HPLC
Selection of the Mobile Phase
Mobile Phase Organic Solvent Percentage %
Temperature
Flow Rate
Wavelength

23. List of PPP compounds, chemical structures and abbreviations

24. Purification and Separation of Priority Pollutant Phenols (PPP) by HPLC
Selection of the Mobile Phase
Organic Solvent Modifier Percentage %
pH of the Aqueous Part of the Mobile Phase
Flow rate
Temperature
Wavelength

25. Method Validation Linearity Limit of Detection (LOD)
Limit of Quantization (LOQ)
Precision and accuracy
Ruggedness and robustness

26. Extraction of the desired component by Solid Phase Extraction (SPE)

27. Extraction of the desired component by Solid Phase Extraction (SPE)
Cartridge Resin
Elution Solvent
pH Effect of Primary Standard Solution

28. Application of Real Samples
Petroleum Refinery Industrial Waste-Water Treatment Stages
Stages of waste-water treatment in the petroleum refinery station with sampling sites A, B, and C.

29. Sampling sites D, E, and F at Tigris River

30. Treatment Study
Experimental protocol for the enzymatic treatment of phenol and benzidine
Batch reactors containing buffer, substrate, hydrogen peroxide, and SBP in a total volume of 20 ml were stirred by magnetic stirrers. The mixture was added in the following order: distilled water, buffer, enzyme, and H2O2of a proper concentration to start the reaction. The mixture was stirred gently for 3 hrs in open air, and then excess catalase enzyme was added quench the reaction after 3 hrs.

31. Optimization of the Removal of Benzidine from Synthetic Waste-Water
Optimum pH
Minimum SBP concentrations
Optimum hydrogen peroxide-to-substrate concentration ratio
Effect of Reaction Time

32. Optimization of Removal of Phenol from Synthetic Waste-Water
Optimum pH
Minimum Enzyme Activity
An optimum hydrogen peroxide -to-substrate concentration ratio

34. Thanks for listening