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Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway.

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Presentation on theme: "Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway."— Presentation transcript:

1 Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway in cultured human chondrocytes  T. Saito, K. Nishida, T. Furumatsu, A. Yoshida, M. Ozawa, T. Ozaki  Osteoarthritis and Cartilage  Volume 21, Issue 1, Pages (January 2013) DOI: /j.joca Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 RT-PCR results showed changes in COL2A1, RUNX-2, ADAMTS-5, and MMP-3 gene expression with various concentrations of TSA (A) and MS-275 (B) in human chondrocytes. After CTS, the expressions of RUNX-2, ADAMTS-5, and MMP-3 were upregulated without HDAC inhibitors, but were not upregulated in the presence of HDAC inhibitors. In contrast, COL2A1 was suppressed after CTS without HDAC inhibitors, but not suppressed with TSA (10, 100, 500nM) or MS-275 (100nM). The PCR amplifications were repeated at least five times and similar results were acquired. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 The effects of HDAC inhibitors (TSA: 10nM, MS-275: 100nM) on COL2A1, RUNX-2, ADAMTS-5, MMP-3 and MMP-13 expression in human chondrocytes. Changes in COL2A1 mRNA expression after 10% mechanical stretch in human chondrocytes (A). Real-time PCR analyses (n=6 per experimental group) showed that COL2A1 mRNA expression decreased 15h after CTS without HDAC inhibitors. In the presence of HDAC inhibitors, COL2A1 mRNA expression after CTS decreased relative to CTS (−), however, the expression increased relative to the absence of HDAC inhibitors. After CTS, the expression of RUNX-2, ADAMTS-5, and MMP-3 was upregulated without HDAC inhibitors and was not upregulated with HDAC inhibitors (B, C, D). MMP-13 expression tended to increase with CTS and decrease with HDAC inhibitor treatment (E). Relative mRNA levels of each gene were normalized to G3PDH controls and were plotted relative to CTS (−) and HDAC inhibitors (−). The data are presented as the mean with 95% CI. *P<0.05, **P< §P=0.10, §§P=1.00. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

4 Fig. 3 The effects of mechanical stress and HDAC inhibitors on protein expressions of RUNX-2 and ADAMTS-5 in human chondrocytes. RUNX-2 expression was upregulated and localized to the nucleus following CTS without HDAC inhibitors (green signals). ADAMTS-5 expression was upregulated and localized in the cytoplasm after CTS without HDAC inhibitors (green signals). These CTS-induced protein expression were suppressed by both TSA (10nM) and MS-275 (100nM). Merged images with the staining by phalloidin and Hoechst were shown in the right-side panels. Cells incubated without primary antibodies were used as controls (lower panel) (A). Arrows denote the direction of stretching. Scale bar=100μm. The cell count data via immunocytochemistry is shown in (B). The results are expressed as a percentage of RUNX-2 or ADAMTS-5 positive cell ratios (number of positive cells/all cells). *P<0.01, relative to other groups. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

5 Fig. 4 Western blot analysis showed RUNX-2 phosphorylation 30min after CTS in human chondrocytes. Total protein extracts were subjected to SDS-PAGE (10mg of total protein/lane), and western blot analysis was performed with total and phosphorylated RUNX-2 (P-RUNX-2) antibodies. RUNX-2 expression was upregulated without HDAC inhibitors, but was not upregulated with HDAC inhibitors. The data are presented as the mean with 95% CI of six determinations. *P<0.001. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

6 Fig. 5 The western blots were probed for total JNK and phosphorylated JNK (P-JNK) (A), total ERK1/2 and phosphorylated ERK1/2 (P-ERK1/2) (B), or total p38 and phosphorylated p38 (P-p38) (C). The fold increase in phosphorylation based on densitometry analysis of western blots. Results of western blotting and densitometry showed phosphorylation of ERK, JNK, and p38 in human chondrocytes after CTS for 30min. Total protein extracts were subjected to SDS-PAGE (10mg of total protein/lane). The data were presented as the mean with 95% CI of six determinations. *P< §P=0.158, §§P=0.534. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

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