1. 3-Valve Pumping Sequence 4-Valve Pumping Sequence
Instrumented Nanophysiometer for High Throughput Drug Delivery D. Michael Ackermann1, Jonathan Payne1, Hilary Samples1, James Wells1 Advisors: Franz Baudenbacher, Ph.D.2; Paul King, Ph.D.1 1 Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 2 Department of Living State Physics, Vanderbilt University, Nashville, TN
Objective
Device
Software Controller
Design a nano-liter sized nanophysiometer suitable for cell culturing
Implement on-chip peristaltic pumps for low volume flow through perfusion
Incorporate thin film microelectrodes in the inflow and outflow channels for monitoring physiological parameters
Develop a LabView user interface to manage valve sequencing
Soft Lithography
[1] S.R. Quake and A. Scherer, "From Micro to Nano Fabrication with Soft Materials", Science 290: (2000).
[2] M.A. Unger, H.-P. Chou, T. Thorsen, A. Scherer, and S.R. Quake, "Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography", Science 288: (2000).
Results
0.51 mm d
Peristaltic Pumping
Future Work
Valve
Flow Direction 1 2 3
Stage
3-Valve Pumping Sequence 4
Closed Valve
Open
Valve
Flow Direction 1 2 3
Stage
4-Valve Pumping Sequence 4
Pump Sequencing (# of valves)
Cells
Electrodes
pH Monitoring
Use integrated thin film microelectrodes to monitor physiological parameters
pH, glucose, etc.
Electrodes coated with a substrate specific oxidase
Catalyze reaction producing H2O2
H2O2 then detected
Acknowledgments