1. BIBC 102
Metabolic Biochemistry
Randy Hampton

2. Ask… or
txt:

3. The isoprene unit:
a branched lipid structure

4. fig 21-32

5. stage 1
mevalonate
production
fig

6. stage 1
mevalonate
production
fig

7. stage 1
mevalonate
production
fig

8. stage 1
mevalonate
production
fig

9. first steps
of ketone
body
formation
synthesis of
mevalonate
fig

10. stage 2: conversion to
active isoprenes
fig 21-35

11. condensation of isoprenes
“tail”
“head”
fig

12. further condensation of isoprenes
fig

13. and even more condensation of isoprenes
fig

14. from squalene to cholesterol
fig

15. fig

16. LDL:
the
infamous
lipoprotein
fig

17. fig 21-39

18. fig 21-40

19. fig 21-40

20. regulation
of cholesterol
metabolism
both uptake
and synthesis
are involved
fig 21-44

21. As does nature, so does pharma...
box 21-3

22. sterols
galore!
fig 21-45

23. isoprenoids
galore!!
fig 21-47

24. Ask… or
txt:

25. Clicker!!

26. Gluconeogenesis
A) refers to synthesis of glucose
B) produces ATP
C) occurs when insulin is high
D) only occurs in plants

27. Fructose-2,6bP
A) is increased when glucose is low
B) increases the rate of gluconeogenesis
C) is a glycolytic intermediate
D) is made by FPK2

28. Fatty Acid Synthesis
A) requires carboxylation of AcCoA
B) produces NADH and FADH2
C) uses NADPH for reductions
D) Both A and C

29. The Glyoxylate Cycle
A) releases CO2
B) is a catabolic cycle
C) occurs in the human liver
D) makes a 4C product from a 2C substrate

30. Glycogen: the glucose buffer…
XXXX

31. Glycogen granules
~ 1600 glucose monomers per granule

32. fig 7-14

33. Glycogen in real life…

34. How is glycogen synthesized?
-sequential addition of glucose to a
growing a1-4 linked linear polymer
glycogenin, then glycogen synthase
-creation of branches composed of a1-6
linkages
branching enzyme

35. Glucose ends… O 1 2 3 4 5 6
non-
reducing
end
reducing
end

36. polymer ends…
fig 7-14

37. pg 597

38. addition of UDP carrier to glucose-1-P
fig 15-29

39. addition of glucose monomers to the growing glycogen chain
fig 15-30

40. Glycogen branching enzyme creates branches
fig 15-31

41. Glycogen branching enzyme creates branches10
a1 4 3 7
a1 6
fig 15-9

42. How is glycogen broken down?
-sequential removal of glucose-1P from
the non-reducing end
glycogen phosphorylase
-dismantling of those pesky branches
debranching enzyme

43. fig 14-11

44. debranching
enzyme
removes
branches
fig 15-26

45. 7 4 7 1 Debranching enzyme removes branches a1 4 a1 6 a1 4 a1 4 a1 6
free
glucose 4
a1 6
a1 4 7
a1 4 1
a1 6
transferase
activity
a1-6
glucosidase

46. Ask… or
txt:

47. Hormonal control of glycogen phosphorylase
fig 15-34

48. Allosteric control of glycogen phosphorylase
fig 15-36

49. regulation of
glycogen
synthase
by phos-
phorylation
as well…
fig 15-37

50. Liver glucose
metabolism
fig 23-13

51. Blood glucose
level is critical
and stringently
regulated
fig 23-23

52. The
Cori
cycle
fig 23-20

53. Liver as a "glucostat"
low blood
glucose
high blood
glucose

54. Glucagon: the hormone of starvation
phosphorylation
of enzymes
alteration of
fructose 2,6 bis-P

55. Insulin: the hormone of plenty

57. fig 23-24

58. b cell glucose sensing: an ATP gated channel