1. driving neuroinflammation into neuroregeneration
prof. Peter Ponsaerts
Laboratory of Experimental Hematology
Neuroinflammation/regeneration group
driving neuroinflammation
into neuroregeneration

2. Cuprizone (CPZ) Mouse Model
for imaging and study of neuroinflammation/regeneration
straightforward MRI and histological analyses
complex neuroinflammation/regeneration pathways
Praet et al. Neuroscience and Biobehavioral Reviews 2014.

3. for in vivo modulation of neuroinflammation
Interleukin 13
for in vivo modulation of neuroinflammation
targeted delivery of interleukin 13 to the brain or spinal cord,
by means of lentiviral vector injection or implantation of genetically engineered stem cells,
reduces inflammation and prevents demyelination
Hoornaert et al. Stem Cells 2016, Le Blon et al. Journal of Neuroinflammation 2016, Guglielmetti et al. Glia 2016, Dooley et al. Stem Cell Reports 2016

4. Decellularised Brain Tissue Scaffolds
for growth and differentiation of neural stem cells
decellularised brain tissue scaffolds for 3D growth of neural stem cells
non-invasive monitoring by means of bioluminescence imaging (luciferase) and fluorescence microscopy (eGFP)
De Waele et al. Biomaterials 2015.

5. iPSC-derived Microglia
for in vitro study and modulation of neuroinflammation
MCAO stroke lesion in
CX3CR1eGFP/+CCR2RFP/+ reporter mouse model
iPSC colonies
DAPI - DPPA4
MEF isolation
Sox2, Oct4, Klf4
microglia differentiation
astrocytes - microglia
CD 45
eGFP
differentiation of eGFP+RFP- microglia
from CX3CR1eGFP/+CCR2RFP/+ iPSC
in co-culture with astrocytes
Quarta et al. manuscript in preparation

6. neuroinflammation/regeneration group
Acknowledgments
neuroinflammation/regeneration group
prof. Zwi Berneman
prof. Peter Ponsaerts
dr. Debbie Le Blon
dr. Chloé Hoornaert
Alessandra Quarta
Evi Luyckx
Elise Van Breedam
Jasmijn Daans