1. TCR‐mediated IL‐2 promoter activation in J.Vav1 cells.
TCR‐mediated IL‐2 promoter activation in J.Vav1 cells. Cells were co‐transfected with 10 μg of pIL2‐luc DNA, 2 μg of pRL‐TK DNA and, where indicated, 4 μg of Vav‐1‐encoding plasmid DNA. After 18 h, the transfected cells were left unstimulated (open bars), or were stimulated with Raji B cells in the absence (horizontal bars) or presence (hatched bars) of superantigen D (SED). The pIL2‐Luc (firefly luciferase) activities were measured and were normalized to the pRL‐TK‐derived Renilla luciferase activity in each sample. Data are presented as the mean normalized relative light units (RLU) from triplicate samples.
Youjia Cao et al. EMBO J. 2002;21:
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