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Chapter 14
Author: Kelly Elkins
© 2013 Elsevier, Inc. All rights reserved.

2. © 2013 Elsevier, Inc. All rights reserved.
Figure bp amplicon produced by PCR primers for mtDNA SNP (bold). Sequence is rCRS (NCBI Accession: NC_012920). Primers are underlined.
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Figure 14.2 Exponential amplification curves that show the results of forty cycles of RT-PCR using the mtDNA SNP primers depicted in Table Curves of standard DNA including K562, 9947A, NIST SRM 2372, all at 1 ng template concentration, and an unknown as detected by SYBR green I.
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Figure A. Melting curve plot from °C showing the temperature inflection at 85.5 °C (left). B. First derivative plot of the melting curve from °C showing the predominant peak at 85.5 °C for the K562, 9947A, NIST SRM 2372, and a unknown sample DNA (right image). All standards used were 16519C allowing the unknown to be assigned as 16519C.
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5. © 2013 Elsevier, Inc. All rights reserved.
Figure 14.4 Sample 2% agarose gel of PCR of K562 DNA using primers for mtDNA SNP 16519T/C. Lanes 2-5 contain the samples with the amplicon and Lane 5 shows the DNA Logic Ladder (sizes indicated).
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