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Hsin-Fu Chen, M. D. , Jin-Yuh Shew, Ph. D. , Hong-Nerng Ho, M. D

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Presentation on theme: "Hsin-Fu Chen, M. D. , Jin-Yuh Shew, Ph. D. , Hong-Nerng Ho, M. D"— Presentation transcript:

1 Expression of leukemia inhibitory factor and its receptor in preimplantation embryos 
Hsin-Fu Chen, M.D., Jin-Yuh Shew, Ph.D., Hong-Nerng Ho, M.D., Wei-Li Hsu, M.S., Yu-Shih Yang, M.D., Ph.D.  Fertility and Sterility  Volume 72, Issue 4, Pages (October 1999) DOI: /S (99)

2 FIGURE 1 Agarose gel electrophoresis of polymerase chain reaction (PCR) amplification products of β-actin, leukemia inhibitory factor (LIF), and leukemia inhibitory factor receptor (LIF-R) complementary DNA from a human two-cell embryo. The PCR product was size-fractionated on a 1% agarose gel stained with SYBR green stain. The signal intensity was determined using a phosphoimage scanner. The sizes of the products were 250, 560, and 459 base pairs (bp), respectively, for β-actin, LIF, and LIF-R. M = molecular weight marker. Chen. Leukemia inhibitory factor. Fertil Steril 1999. Fertility and Sterility  , DOI: ( /S (99) )

3 FIGURE 2 Agarose gel electrophoresis of polymerase chain reaction (PCR) amplification products of β-actin, leukemia inhibitory factor (LIF), and leukemia inhibitory factor receptor (LIF-R) complementary DNA from a murine two-cell embryo and a blastocyst. The PCR product was size-fractionated on a 1% agarose gel stained with SYBR green stain. The signal intensity was determined using a phosphoimage scanner. The sizes of the products were 250 and 316 base pairs (bp), respectively, for β-actin and LIF-R. In this specific study, LIF products were not detectable in either embryo. M = molecular weight marker. Chen. Leukemia inhibitory factor. Fertil Steril 1999. Fertility and Sterility  , DOI: ( /S (99) )


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