1. The Polymerase Chain Reaction (PCR): Replicating DNA in the Test Tube
Day 1

2. Review DNA Replication
Read and study Textbook Appendix C Genetics, pages A 24 & A 25

3. VNTR Polymorphism For this experiment: pMCT118 of chrom. 1
Most people have between copies (calculate possible fragment size!)
The different versions are called alleles
How many alleles do you have?
pMCT118 consists of 16 bp repeat unit

4. Example:

5. Ingredients needed Target DNA Chelex Taq polymerase Primers Four dNTPs
Mg2+ (function?)

6. PCR Procedure One cycle has 3 steps: Denaturation Annealing Extension
Approximately 30 cycles are needed

7. PCR Overview
PCR Animations
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