Presentation is loading. Please wait.

Presentation is loading. Please wait.

الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي:

Published byEsmond Wheeler Modified over 6 years ago

Similar presentations

Presentation on theme: "الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي:"— Presentation transcript:

1 الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي:
الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي: Components Stock concentration Final concentration Template 125 ng/μL 7.5 ng/μL Primer F 200 ng/μL 4.0 ng/μL Primer R dNTP mix 2.0 mM each 0.2 mM PCR buffer 10 X 1 X MgCl2 25 mM 1.5 mM Taq polymerase 5 U/μL 0.05 U/μL H2O

2 الكميات المطلوبة لتحضير 20 ميكروليتر الحجم الكلي للتفاعل هي:
الكميات المطلوبة لتحضير 20 ميكروليتر الحجم الكلي للتفاعل هي: Components Stock concentration Final concentration Template 125 ng/μL 7.5 ng/μL Primer F 200 ng/μL 4.0 ng/μL Primer R dNTP mix 2.0 mM each 200 μM PCR buffer 10 X 1 X MgCl2 25 mM 1500 μM Taq polymerase 5 U/μL 0.05 U/μL H2O

3 ما هو الحجم المطلوب إضافته إلى برايمر وزنه 23 نانو مول ليكون تركيزه النهائي 100 ميكرو مولر

Download ppt "الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي:"

Similar presentations

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)
PCR way of copying specific DNA fragments from small sample DNA material molecular photocopying It’s fast, inexpensive and simple Polymerase Chain Reaction.

PCR way of copying specific DNA fragments from small sample DNA material "molecular photocopying" It’s fast, inexpensive and simple Polymerase Chain Reaction.
COMPUTER EXERCISE Design of PCR and PCR-RFLP experiments This presentation shows all steps of a PCR-RFLP experiment and is a companion of the computer.

COMPUTER EXERCISE Design of PCR and PCR-RFLP experiments This presentation shows all steps of a PCR-RFLP experiment and is a companion of the computer.
PCR and Forensics YouTube - CSI Intro YouTube - CSI Intro.

PCR and Forensics YouTube - CSI Intro YouTube - CSI Intro.
PCR Basics Purpose of PCR Overview Components of PCR Reaction

PCR Basics Purpose of PCR Overview Components of PCR Reaction
PCR Basics 1.Purpose of PCR 2.Overview 3.Components of PCR Reaction 4.Variables Temperature Cycle Times and Numbers Primer Buffer Polymerase 5.Experimental.

PCR Basics 1.Purpose of PCR 2.Overview 3.Components of PCR Reaction 4.Variables Temperature Cycle Times and Numbers Primer Buffer Polymerase 5.Experimental.
PCR Polymerase Chain Reaction. PCR - a method for amplifying (copying) small amount of DNA in nearly any amount required, starting with a small initial.

PCR Polymerase Chain Reaction. PCR - a method for amplifying (copying) small amount of DNA in nearly any amount required, starting with a small initial.
Tucson High School Biotechnology Course Spring 2010.

Tucson High School Biotechnology Course Spring 2010.
Chapter 8 Mutagenesis of cloned DNA. To creating numerous mutations in a small DNA sequence, mutagenesis with degenerate oligonucleotides a palindromic.

Chapter 8 Mutagenesis of cloned DNA. To creating numerous mutations in a small DNA sequence, mutagenesis with degenerate oligonucleotides a palindromic.
Techniques Making a Crude Cell Extract. Why bother understanding how a technique works? Why not just do it? Because understanding: n Minimizes performance.

Techniques Making a Crude Cell Extract. Why bother understanding how a technique works? Why not just do it? Because understanding: n Minimizes performance.
1. Sample & Primer 2009. 03. 23 CEDDGP001- 카톨릭의대 정의달 * 총 10 개 샘플중에서 4, 14, 24, 28, 36 샘플에 대해서만 PCR 실험을 수행하였다. PCR 실험 수행시 DNA 농도를 100ng/ul 로 희석하여 사용하였다.

1. Sample & Primer CEDDGP001- 카톨릭의대 정의달 * 총 10 개 샘플중에서 4, 14, 24, 28, 36 샘플에 대해서만 PCR 실험을 수행하였다. PCR 실험 수행시 DNA 농도를 100ng/ul 로 희석하여 사용하였다.
POLYMERASE CHAIN REACTION (PCR). THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS.

POLYMERASE CHAIN REACTION (PCR). THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS.
PCR of our OV What is PCR again? I forgot… Preparing for PCR Running a PCR.

PCR of our OV What is PCR again? I forgot… Preparing for PCR Running a PCR.
PCR Troubleshooting.

PCR Troubleshooting.
©2001 Timothy G. Standish Romans 5:17 17For if by one man’s offence death reigned by one; much more they which receive abundance of grace and of the gift.

©2001 Timothy G. Standish Romans 5:17 17For if by one man’s offence death reigned by one; much more they which receive abundance of grace and of the gift.
Lab safety Documentation, GLP Practical tips; primers and PCR.

Lab safety Documentation, GLP Practical tips; primers and PCR.
PCR Troubleshooting Virginia Balke

PCR Troubleshooting Virginia Balke
Speciation of Methicillin-Resistant Staphylococci Isolated from Ecuadorian Hospitals and Communities Student Researcher: Beatrice R. Soderholm; Faculty.

Speciation of Methicillin-Resistant Staphylococci Isolated from Ecuadorian Hospitals and Communities Student Researcher: Beatrice R. Soderholm; Faculty.
Chapter 14: DNA Amplification by Polymerase Chain Reaction.

Chapter 14: DNA Amplification by Polymerase Chain Reaction.
Polymerase Chain Reaction (PCR) Developed in 1983 by Kary Mullis Major breakthrough in Molecular Biology Allows for the amplification of specific DNA fragments.

Polymerase Chain Reaction (PCR) Developed in 1983 by Kary Mullis Major breakthrough in Molecular Biology Allows for the amplification of specific DNA fragments.

Similar presentations

Ads by Google