1. Lab Safety

2. No food, drinks, gum, or candy in the lab.

3. General Safety NO HORSEPLAY!!! Be careful not to bump into others.
Learn the location of safety equipment:
Eyewash station – sinks 1 and 5, adjacent science lab
Fire Blanket – near sink 5
First Aid Kit – near sink 5
Safety Shower – adjacent science lab
Fire Extinguisher – hallway between classroom and adjacent science lab, hallway near classroom entrance

4. Directions
Read ALL directions and make sure you understand prior to beginning an activity.
Do not begin until the teacher has instructed you to do so.
Never experiment on your own.
Tell your teacher immediately if you are injured.

5. Glassware Safety Never use broken or chipped glassware.
Notify your teacher of broken glass.
Never heat glassware that
is not thoroughly dry.
Never pick up glassware unless you are certain it is not hot. If it is hot, use heat-resistant gloves or tongs.
Do not put hot glassware in cold water.

6. Sharp Instrument Safety
Always use single-edged razors.
Handle sharp instruments with caution.
Never cut any material toward you.
Dispose of used or ruined sharp instruments in a container specified by your teacher.
Carry a scalpel in a tray
or with tip down.

7. Fire and Heat Safety
Never use an open flame without wearing safety goggles.
Never heat anything unless instructed to do so.
Never heat anything in a closed container.
Never leave a test tube directly in a flame.
Never reach across a flame.
Always use a clamp, tongs, or heat-resistant gloves to handle hot objects.

8. Animal Safety Do not cause pain,
discomfort, or injury to a live animal.
Follow your teacher’s directions when handling animals.
Wash your hands thoroughly after handling animals or their cages.
Treat animal specimens with respect.

9. Electrical Safety
Never use a long extension cord to plug in an electrical device.
Do not plug too many appliances into one socket.
Never touch an electrical appliance or outlet with wet hands.
Unplug equipment at the
end of the lab activity.

10. Chemical Safety Always wear goggles, safety apron, and gloves when
handling chemicals.
If a chemical contacts your skin, rinse immediately and notify the teacher.
Never smell any chemicals directly from the container.
Waft only when instructed to do so.
Never mix chemicals unless instructed to do so.

11. Chemical Safety Keep all lids closed when chemicals are not in use.
Dispose of all chemicals as instructed by
your teacher.

12. Eye and Face Safety Wear goggles when handling chemicals.
When heating a test tube or bottle, always point it away from you and others.
Remember: chemicals can splash or boil out of a heated test tube.
If a chemical comes in contact with your eyes, use the eyewash fountain immediately and seek emergency care. Flush eyes for at least 15 minutes.

14. Proper Dress Tie back long hair. Remove or tie back dangling jewelry.
Ideally, lab clothes should
consist of fitted long
sleeves, pants or
long dress, and
closed-toed shoes.

15. Clean Up Keep work area uncluttered.
Follow the teacher’s instructions for throwing away or putting away supplies.
Clean your work area.
Wash your hands.

16. If it seems like it is not a good idea,
USE COMMON SENSE
If it seems like it is not a good idea,
it probably is not!!!

18. Beakers Use to mix, measure, or heat liquids They are NOT designed
for accurate measurement
Use a beaker that holds
about twice as much
liquid as you need

19. Test Tubes Use to hold small amounts of substances
Use to mix or heat liquids
DO NOT use to measure the volume of a liquid – they are not
usually marked with
measurements

20. Test-Tube Holder
Use when heating a substance in a test tube

21. Test-Tube Rack
Used to keep test tubes upright so they do not fall over and spill their contents

22. Graduated Cylinder
“Graduated” or marked with a scale for measurement. Used to accurately measure liquid volume.

23. Erlenmeyer Flask
Used to measure liquid – not as accurate as graduated cylinder
Helps prevent liquids from splashing or giving off noxious fumes

24. Pipette
Used to dispense small measures of liquid

25. Thermometer
Used to measure temperature

26. Petri Dish
Used to culture (grow) bacteria and other microorganisms on a nutrient agar

27. Tongs
Used to grasp heated materials

28. Watch Glass
Serves as a holding container

29. Hot Plate
Source of electrical heat

30. Bunsen Burner
Source of gas heat

31. Tripod Holds glassware above a Bunsen burner
Piece of wire gauze sits on top of tripod to hold the container

32. Triple-Beam and Mechanical Balance
Used to determine the mass of materials to the nearest gram or ten thousandth of a gram

33. Mechanical Pan Balance
Used to accurately determine mass to the nearest ten thousandth of a gram

34. Spring Scale
Used to determine force

35. Chemical Fume Hood
Used to contain and safely remove hazardous gases from the laboratory

36. Microscope
Allows for visualizing very small objects or organisms too small to see with the unaided eye

37. Telescope
Allows visualization of objects too far away to be seen with the unaided eye

38. Meter Stick
Measures length or width

39. Scalpel
Sharp bladed instrument for cutting

40. Tweezers / Forceps
Grasping

41. Probe
Blunt-ended instrument for exploring specimen

42. Teasing Needle
Tapered end used for separating tissues

43. Scissors
Cutting