1. Experiment Questions
General Expts

2. General Expts
For what purpose did you use iodine in the course of your practical studies? To stain plant cells / test for starch

3. General Expts
State: (i) An investigation in which you used an anaerobic jar or alkaline pyrogallol (ii) The precise purpose for its use in the investigation that you have indicated. (i) To investigate conditions for germination (ii) To remove oxygen

4. General Expts
State: (i) An investigation in which you used cold alcohol (ethanol), (ii) The precise purpose for its use in the investigation. (i) isolation of DNA (ii) to separate DNA

5. General Expts
For what purpose did you use freezer-cold alcohol in the course of your practical activities? To bring DNA out of solution / to isolate DNA

6. General Expts
What is meant by sterile? Free from (micro)organisms or living things or named organism

7. General Expts
How may apparatus be sterilised? autoclave/ heat qualified e.g. high or 100 ºC / boiling / steam / any anti-bacterial agent or named / U.V light

8. General Expts
State two different uses of a water bath in biological investigations. Keep temperature constant (or example) / to vary temperature (or example) / denaturing enzyme / heating

9. General Expts
For what purpose did you use Buffer solution in the course of your practical activities? To keep pH constant

10. General Expts
For what purpose did you use Sodium alginate in the course of your practical activities? To immobilise enzymes (or yeast) / to make beads

11. General Expts
Suggest a reason for using limewater in an experiment. To show the presence / absence of CO2