Presentation is loading. Please wait.

Presentation is loading. Please wait.

Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis  John Backus, Todd Laughlin, Yixin Wang,

Published byAleesha Cole Modified over 6 years ago

Similar presentations

Presentation on theme: "Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis  John Backus, Todd Laughlin, Yixin Wang,"— Presentation transcript:

1 Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis  John Backus, Todd Laughlin, Yixin Wang, Robert Belly, Robert White, Jon Baden, C. Justus Min, Ann Mannie, Lorraine Tafra, David Atkins, Kathryn M. Verbanac  The Journal of Molecular Diagnostics  Volume 7, Issue 3, Pages (August 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Marker selection process. Identification of breast-specific markers and/or breast cancer-status markers was carried out using a series of four filters: 1) expression in WBCs, 2) expression level in breast cancer tissues, 3) expression level in normal/benign breast tissues, and 4) expression in other epithelial cancers. Markers had to pass the first two criteria and either of the last two criteria to be selected. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Sensitivity of markers in lymph nodes. Individual marker sensitivity was assessed in lymph node tissues by RT-PCR. A specificity of 94% was used to identify individual markers that offered optimal performance for detection of clinically actionable metastases. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Sensitivity of marker combinations in lymph nodes. Two-marker and three-marker combinations were assessed in lymph node tissues by RT-PCR. A specificity of 94% was used to identify marker combinations that offered optimal performance for detection of clinically actionable metastases. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis  John Backus, Todd Laughlin, Yixin Wang,"

Similar presentations

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,

Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,
A Multiplex qPCR Gene Dosage Assay for Rapid Genotyping and Large-Scale Population Screening for Deletional α-Thalassemia Wanjun Zhou, Ge Wang, Xuefeng.

A Multiplex qPCR Gene Dosage Assay for Rapid Genotyping and Large-Scale Population Screening for Deletional α-Thalassemia Wanjun Zhou, Ge Wang, Xuefeng.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
Telomerase Activity and Expression of Telomerase RNA Component and Telomerase Catalytic Subunit Gene in Cervical Cancer Kenji Nakano, Elizabeth Watney,

Telomerase Activity and Expression of Telomerase RNA Component and Telomerase Catalytic Subunit Gene in Cervical Cancer Kenji Nakano, Elizabeth Watney,
Molecular Testing Guideline for Selection of Lung Cancer Patients for EGFR and ALK Tyrosine Kinase Inhibitors Neal I. Lindeman, Philip T. Cagle, Mary Beth.

Molecular Testing Guideline for Selection of Lung Cancer Patients for EGFR and ALK Tyrosine Kinase Inhibitors Neal I. Lindeman, Philip T. Cagle, Mary Beth.
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling Anna E. Szafranska, Timothy S. Davison,

Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling Anna E. Szafranska, Timothy S. Davison,
Transgenic Mice Overexpressing BMP4 Develop a Fibrodysplasia Ossificans Progressiva (FOP)-Like Phenotype Lixin Kan, Min Hu, William A. Gomes, John A. Kessler.

Transgenic Mice Overexpressing BMP4 Develop a Fibrodysplasia Ossificans Progressiva (FOP)-Like Phenotype Lixin Kan, Min Hu, William A. Gomes, John A. Kessler.
Duplex Ratio Tests as Diagnostic Biomarkers in Malignant Melanoma

Duplex Ratio Tests as Diagnostic Biomarkers in Malignant Melanoma
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.
Droplet Digital PCR for Absolute Quantification of EML4-ALK Gene Rearrangement in Lung Adenocarcinoma  Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin,

Droplet Digital PCR for Absolute Quantification of EML4-ALK Gene Rearrangement in Lung Adenocarcinoma  Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin,
Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood  Michael Mitas, Loretta Hoover, Gerard Silvestri,

Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood  Michael Mitas, Loretta Hoover, Gerard Silvestri,
A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin  Dimitri Talantov, Jonathan Baden,

A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin  Dimitri Talantov, Jonathan Baden,
Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,
Eligibility Criteria and Genetic Testing Results from a High-Risk Cohort for Hereditary Breast and Ovarian Cancer Syndrome in Southeastern Ontario  Ricardo.

Eligibility Criteria and Genetic Testing Results from a High-Risk Cohort for Hereditary Breast and Ovarian Cancer Syndrome in Southeastern Ontario  Ricardo.

Similar presentations

Ads by Google