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SCIENCE VOL 301 26 SEPTEMBER 2003 Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins Jwa-Min Nam,* C. Shad Thaxton,* Chad A.

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Presentation on theme: "SCIENCE VOL 301 26 SEPTEMBER 2003 Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins Jwa-Min Nam,* C. Shad Thaxton,* Chad A."— Presentation transcript:

1 SCIENCE VOL SEPTEMBER 2003 Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins Jwa-Min Nam,* C. Shad Thaxton,* Chad A. Mirkin Qing Han Presentation

2 Background PCR Immuno-PCR ELISA Sensitivity~pM

3 Drawbacks of current immuno-PCR
A low ratio of DNA to detection Ab Slow target-binding kinetics Link the Ab and DNA markers PCR requirements

4 The bio-bar-code assay method -----Probe designand preparation.
Detection Ab Capture Ab 1 um

5 PSA detection and bar-code DNA amplification and identification

6 Scanometric detection of PSA-specific bar-code DNA

7 Bio–bar-code method offers several advantages
Homogeneous target-binding portion High ratio of PCR-amplifiable DNA to Ab No need for complicated conjugation chemistry Low background signal potential for the simultaneous detection of many analytes in one solution

8 Questions?

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