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International Journal of Surgery
Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability Mohammad Reza Farahpour, Navideh Mirzakhani, Jamal Doostmohammadi, Mahmood Ebrahimzadeh International Journal of Surgery Volume 17, Pages (May 2015) DOI: /j.ijsu Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 1 Histological feature of experimentally-induced wound on 3 after surgery. A) control-sham, B) 1.5% pistachio-treated, C) 3% pistachio-treated and D) 5% pistachio-treated. Note the higher magnifications for granulation tissue; Well formed granulation tissue and decreased edema is presented in 5% pistachio-treated group, (Masson-trichrome staining, 400× & 600×). FN: fibrinoid necrosis, ED: edema, S: scab. International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 2 Histological feature of experimentally-induced wound on 14 after surgery. A) control-sham, B) 1.5% pistachio-treated, C) 3% pistachio-treated and D) 5% pistachio-treated. Note the newly generated epidermis in control-sham that is completed in 3 and 5% pistachio-treated group. Faint edema remained in deeper dermis of the 1.5% pistachio-treated group, while is significantly limited in 5% pistachio-treated group. Complete layers of the epidermis are generated in 3 and 5% pistachio-treated groups. See the regular basement membrane cells and the spinosume cellular layer. Papilla is completely generated after 14 days in 5% pistachio-treated group. No edema is detectable in 3 and 5% pistachio-treated groups, (Masson-trichrome staining, 400× & 600×). EP: epidermis, De: dermis, D.De: deeper dermis, BL: basement layer, Sp: spinosume stratum, Kr: keratin. International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 3 Histological feature of experimentally-induced wound on 21 after surgery. Fluorescent staining for RNA and Collagen with RGB image plot for red and green reactions in 600 μm for A) control-sham, B) 1.5% pistachio-treated, C) 3% pistachio-treated and D) 5% pistachio-treated. Note increased collagen synthesis accomplished with enhanced RNA content in 3 and 5% pistachio-treated groups. E: epithelium, Co: collagen, F: hair follicle. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 4 Micro vessels distribution per one mm2 of the tissue in different days and different administrated doses, all data are presented in Mean ± SD. Note: a, b, c, d, e, f are presented significant differences between marked groups. International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 5 Mast cells distribution in different groups; PAO enhanced mast cells distribution more prominently on day 7 after wound induction. All data are presented in Mean ± SD and P < 0.05 considered as significant difference. Note: a, b, c presented significant differences between marked data. International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 6 Fluorescent photomicrograph for mast cells distribution 7 days after wound induction; A) control, B) 1.5% PAO-treated, C) 3% PAO-treated and D) 5% PAO-treated. Note dose dependently increased mast cell distribution (head arrow) enclosed to vessels (arrows) in treated groups, (Fluorescent staining for mast cells, 400×). International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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Fig. 7 Pistachio atlantica hulls hydroethanolic extract-induced early wound healing process. International Journal of Surgery , 88-98DOI: ( /j.ijsu ) Copyright © 2015 IJS Publishing Group Limited Terms and Conditions
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