1. Today:
Ionic Equilibria Wrap- up
Recombinant DNA
and Biotechnology

2. Next Up: DNA Technology
What’s interesting, exciting, frightening?
Capture your thoughts: Question 1

3. DNA Tools: Restriction Enzymes
What do Restriction Enzymes do?
Where do they cut (and why)??

4. DNA Tools: Restriction Enzymes

5. DNA Tools: Gel Electorphoresis
You Try! Question 2

6. DNA Tools: Southern Blots
You Try! Question 3

7. DNA Tools: Western and Northern Blots
What can a Northern or Western Blot tell us that a Southern can’t??
You Try: Question 4

8. DNA Tools: Fingerprints
DNA Fingerprints can be made from highly variable regions (polymorphisms).
Two are commonly used: Single Nucleotide Polymorphisms (SNPs) and Short Tandem Repeats (STRs)

9. DNA Tools: Fingerprints
You Try! Question 5

10. DNA Tools: Barcodes?!?

11. Building Recombinant DNA

12. DNA Tools: Vectors Good Vectors: Replicate Independently
Provide Recognition Sequences for Restriction Enzymes
Include a Reporter Gene
Are Small

13. Where do you get DNA to Clone??
DNA (Gene) Libraries?
Are all these clones useful to you?

14. How Could You Get a Library of Expressed, Edited Genes?!?

15. DNA Tools: Knock-Outs

16. DNA Tools: Antisense RNA

17. DNA Tools: Micro-arrays
Let’s watch!

18. Applications: Medically Useful Proteins

19. Applications: Medically Useful Proteins

20. Applications: Pharming
Webster and Pete;
Photo BBC

21. Applications: Agriculture

22. Applications: Creating Life?!
Let’s Watch!
Capture your thoughts! Question 6