1. Double‐stranded RNA binding of NS3 is required for HIV‐1 Tat complementation.
Double‐stranded RNA binding of NS3 is required for HIV‐1 Tat complementation. HEK293T cells were transfected with a Tat wild‐type HIV‐1 variant (HIV‐rtTA‐Tatwt) or a Tat negative HIV‐1 variant (HIV‐rtTA‐Tatfs) in combination with Tat, NS3, NS3 mutant (NS3m) or pBluescript (−; 900 ng). CA‐p24 in the culture supernatant was measured at 2 days post‐transfection. The mean of at least three independent experiments is shown with standard error. dsRNA, double‐stranded RNA; HEK, human embryonic kidney; HIV‐1, human immunodeficiency virus type 1; NS3, non‐structural protein 3; Tat, transactivator of transcription.
Esther Schnettler et al. EMBO Rep. 2009;10:
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