1. Effects of cytokines on P450 mRNA expression in human hepatocytes.
Effects of cytokines on P450 mRNA expression in human hepatocytes. Cells were treated with phosphate-buffered saline (1 μl/ml, Control), LPS (10 μg/ml), IL-6 (10 ng/ml)), TNF (10 ng/ml), IFN (10 ng/ml), TGF (10 ng/ml), or IL-1 (5 ng/ml) for 24 h and mRNA levels of CYP2C8 (A), CYP2C9 (B), CYP2C18 (C), CYP2C19 (D), CYP3A4 (E), and CYP2B6 (F) were determined as described under Materials and Methods. All treatments were carried out in triplicate for each individual, and the results are the means ± S.E.M. of nine subjects in each group. Significant differences compared with control: *, p < 0.05; **, p <
Alison E. Aitken, and Edward T. Morgan Drug Metab Dispos 2007;35:
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