1. Lab 3 Connective tissue (2)
Cartilage,Bone and Blood smear
Jun Zhou(周俊）, Ph.D & M.D
School of Medicine,Zhejiang University

2. Slides 1.cartilage ( No.3 trachea) 2.Bone (No.5)
3.Blood smear (picture drawing)

3. Trachea (No.3) Hyaline Cartilage C-shape hyaline cartilage
Location of hyaline cartilage:articular surface of synovial joints, rings of trachea, plates in the bronchi
Trachea (No.3)

4. Hyaline Cartilage Trachea(No.3)：H&E ×40
This a photomicrograph of a routine H&E preparation of hyaline cartilage shows its general features. Note the extensive extracellular matrix that separates a sparse population of chondrocytes.
Resistant to compression, provides cushioning, smooth and low friction surface for joints.
Trachea(No.3)：H&E ×40

5. Trachea：Hyaline Cartilage H&E ×400
capsule
lacuna
perichondrium
The DCT overlying the perichondrium, from which new cartilage cells are derived.
Appositional growth and interstitial growth, the latter is responsible for the formation of isogenous groups.
Mature chondrocytes with clearly visible nuclei reside in the lacunae.
A dark-staining capsule or territorial matrix immediately surrounding the lacunae.
Isogenous groups
Trachea：Hyaline Cartilage H&E ×400

6. Bone Haversian system(osteon) Central canal Circumferential lamella
Interstitial lamella
Bone lacuna
Bone canaliculus
Bone is a sepcialized CT characterized by a mineralized extracellular matrix. Calcium phosphate, in the form of hydroxyapatite crystals, is deposited along the collagen fibrils and in the proteoglycan ground substance. Bone serves as a storage site for calcium and phosphate, which can be released to the blood to maintain homeostatic levels.
During internal remodeling of bone, the involved destruction of osteons left remnants and become the intertitial lamellae.
Silver ×100

7. Bone：Silver ×400 Haversian system(osteon) Central canal Bone lacuna
Bone canaliculus
Osteons are essentially cylindrical structures.The long axes of the osteons are oriented parallel to the long axis of the bone.At the center is osteonal canal.
Because the organic materials is not retained in ground sections,the Harversian canals and other spaces will appear black, as they do here, if filled with air or other dye.
Bone：Silver ×400

8. Bone：Silver ×100 perforating (Volkmann’s) cannals
Blood vessels reach the Haversian canals from the marrow through other tunnels called perforating (Volkmann’s) cannals. In some instances, as here, Volkmann’s canals travel from one Harversian canal to another. Volkmann’s canals can be distinguished from Harversian canals in that they pass through lamellae, whereas Haversian canals are surrounded by concentric rings of lamellae.
Bone：Silver ×100

9. Stained with Wright’s stain.
Blood smear:
A drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide.
Stained with Wright’s stain.
Try to find an area with evenly distributed cells and normal cell shapes.

10. Blood smear：Wright ×400 Erythrocytes: 7-8um Biconcave disc
Stain uniformly with eosin
Neutrophil:
proportion %;
Most numerous
Multilobed nucleus
Pale pink granules
The method of producing a blood smear: a drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide.
A smear from peripheral blood.The cells are mainly erythrocytes.
Blood smear：Wright ×400

11. Eosinophils. Blood smear：Wright ×400 8-10 microns morphology
2-4%
8-10 microns
morphology
bilobed nucleus large,
uniform eosinophil granules.
Blood smear：Wright ×400

12. Blood smear：Basophil Giemsa stain ×400
Basophils.
Proportion 0.5%
morphology - large, irregular basophil granules obscure the nucleus
The basophil granules are very large and irregularly shaped. Granules obscure the nucleus.
Blood smear：Basophil Giemsa stain ×400

13. Blood smear：Wright ×400 Lymphocyte: Intensely stained nucleus
Possess a slight indentation
Small amount of cytoplasm
Pale blue
Blood smear：Wright ×400

14. Monocyte: Blood smear：Wright ×400 nucleus ovoid to kidney shaped
blue-gray Cytoplasm
Blood smear：Wright ×400

15. Platelets: Blood smear：Wright ×400 Small speck-like objectives
Aggregated into small groups
From megakaryocytes in myeloid tissue.
Platelets are indicated by arrows. Some leukocytes are present.
Blood smear：Wright ×400

16. Blood smear：Wright ×400

17. THE END!