1. SURVEY OF BIOCHEMISTRY Protein Function
BEGIN CLASS BY REVIEWING EXAM #1

2. O2 Binding to Hemoglobin
Hill Equation: Derive on board Y
1-Y
= n log (pO2) - n log p50
log
Cooperativity: binding of a ligand to one site affects the binding of other ligands to other sites.
Hill Constant Cooperativity
n = 1 No cooperativity (hyperbolic)
n > 1 Cooperative binding
n < 1 Noncooperative binding

3. Allosteric Interactions
T state without O2
R state with O2
T -> R transition causes structural changes!
See 7-1D

4. Bohr Effect Enhances O2 Transport
As pH  O2 affinity in Hb 

5. Bohr Effect and O2 Transport

6. BPG Binds to T-State of Hb Only

7. Proteins in Muscle Contraction
Thick filament: Myosin
Thin filament:
Actin
Troponin
Ca2+-binding protein
Binds to both tropomyosin and actin
Tropomyosin binds to actin

8. Muscle Protein Structure
Myosin
Complex
Globular head group
Long, fibrous alpha-helical tail

9. Force Generation in Muscle

10. Force Generation in Muscle

11. Force Generation in Muscle

12. Force Generation in Muscle

13. Force Generation in Muscle

14. Force Generation in Muscle

15. Cellular (T cells) Humoral (B cells) Immunity Viruses Fungi Parasites
Foreign Tissue
Humoral (B cells)
Bacteria
Extracellular Viruses
T cells develop in thymus
Antibodies develop in B cells which mature in bone marrow

16. Immunoglobins