1. Cg-OxyR controls catalase transcription, recovery from lag phase, survival to H2O2 stress, and H2O2 scavenging rate.
Cg-OxyR controls catalase transcription, recovery from lag phase, survival to H2O2 stress, and H2O2 scavenging rate. (A) katA mRNA levels under normal conditions in the different C. glutamicumstrains (n = 3). (B) katA mRNA levels in the different C. glutamicum strains after 10 or 30 min upon a 10 mM H2O2 challenge (n = 3). (C) Growth curves for the different C. glutamicum strains (n = 3). (D) In vivo resistance to H2O2. The different Cg-OxyR strains were spotted on agar plates containing increasing concentrations of H2O2, and growth was evaluated. (E) H2O2 consumption rates. An 8-mM (Left) or 40-mM (Right) H2O2 bolus was added to growing cultures of the Cg-OxyR strains, and the H2O2 concentration decrease was monitored in function of time using the FOX reagent (n = 3). Samples were statistically compared with Student’s t test when indicated (NS, not significant; *P < 0.1; **P < 0.05; ***P < 0.01; #P < 0.005).
Brandán Pedre et al. PNAS doi: /pnas
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