1. AIM: HOW DO WE USE MICROSCOPES?
TOPIC: MICRSCOPES
AIM: HOW DO WE USE MICROSCOPES?
DO NOW:
1.Hypothesize……. What are these pictures of?
2. List safety rules needed in order to work with scopes in the lab.

2. 1.)

3. 2. 3.

4. 1. Carrying microscope always use one hand on arm and the other hand under base close to your body
Things to remember when using a microscope

5. 2. DO NOT use the coarse adjustment under high power

6. 3. Always start off with low power
b/c it gives you a greater field of view.

7. 4. Before switching to high power you must: a. focus your image b
4. Before switching to high power you must: a. focus your image b. center your image

8. 5. When cleaning a lens, use LENS PAPER
5. When cleaning a lens, use LENS PAPER. Do not use a napkin, or paper towel…

9. Unstained cheek cells
Stained cheek cells

10. Total magnification
Mathematical calculation that tells you how much larger the specimen is than its actual size
Formula :
Total magnification =
Eyepiece X Objective lens

11. Total magnification=4 x10= 40

12. 100x
400x

13. Total Magnification: Ocular X Objective = Total
(Eyepiece) magnification
Practice:
Eyepiece
Objective
lens
Total
magnification
20x 4x
25x
10x
15x
40x

14. F Image Appearance Enlarged Upside down & backwards On the slide
Seen with naked
eye
Seen through lens F

16. 1. Place a drop of water on the slide Aim: How do I prepare a Wet
Mount
Do Now:
Identify
4 parts of scope

17. 2. Place specimen on the water using tweezers.

18. 3. Slowly lower the cover slip onto specimen on an angle to avoid AIR BUBBLES.

20. Treating a specimen with solutions of different colors to make cell parts more visible
Staining

22. 3. Excess water should be absorbed with a paper towel.

23. Examples:
1. Lugol’s Iodine
Used for plant cells

24. Onion Cells stained with Lugol’s Iodine

25. 2. Methylene blue
Used for animal cells

26. Skin Cells stained with Methylene Blue

28. Total Magnification: Ocular X Objective = Total
(Eyepiece) magnification
Practice:
Eyepiece
Objective
lens
Total
magnification
10x 4x
15x
60x