1. Lyven protocol For each erlen: following of starch hydrolyze
Removal of 0.5 ml of starch solution
Adding 2.5 ml iodine reagent
Mixing by reversal
Read optical density at 630 nm
At the beginning starch is blue dark. Then it turns to brown when it is transformed in dextrin with amylase activity. At the end of hydrolyze the solution stay yellow
Composition

2. Time of hydrolyze with amylase
Starch
Dextrin
Time of hydrolyze with amylase

3. Iodine reagent Iodine concentrated solution: Iodine reagent:
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Iodine concentrated solution:
4.4 g of potassium iodure (KI)
2.2 g of iodine (I2)
Dissolve successively in about 20 ml of distilled water, complete to 100 ml
Store this solution in dark glass maximum one month
Iodine reagent:
Dissolve 4g of potassium iodure in about 80 ml of distillated water
Add 0.4 ml of iodine concentrated solution
Complete to 100 ml with distilled water
Store maximum one day
Individual protection equipment is recommended for iodine handling