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Recombinant lipid transfer protein Cor a 8 from hazelnut

Published byKaren Kennedy Modified over 5 years ago

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Presentation on theme: "Recombinant lipid transfer protein Cor a 8 from hazelnut"— Presentation transcript:

1 Recombinant lipid transfer protein Cor a 8 from hazelnut
Frauke Schocker, PhD, Dirk Lüttkopf, PhD, Stephan Scheurer, PhD, Arnd Petersen, PhD, Anna Cisteró-Bahima, MD, Ernesto Enrique, MD, Mar San Miguel-Moncín, MD, Jaap Akkerdaas, MSc, Ronald van Ree, PhD, Stefan Vieths, PhD, Wolf-Meinhard Becker, PhD  Journal of Allergy and Clinical Immunology  Volume 113, Issue 1, Pages (January 2004) DOI: /j.jaci

2 FIG 1 IgE immunoblotting of hazelnut extract: lanes 1 to 26, patient sera 1 to 26; lane 27, nonallergic control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

3 FIG 2 Nucleotide sequence and deduced amino acid sequence of Cor a 8. The N-terminus determined in nCor a 8 is shown in bold, and the identified signal peptide is underlined. The nucleotide sequence has been submitted to GenBank under accession no. AF Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

4 FIG 3 FPLC purification of recombinant (His10)-tagged Cor a 8 with nickel-nitrilotriacetic acid superflow resin: lane 1, flow-through after binding to column; lane 2, eluates after the 10 mmol/L imidazole binding step; lane 3, eluates after the 50 mmol/L imidazole washing step; lane 4, eluates after the 100 mmol/L imidazole washing step; lanes 5 to 7, elution with 250 mmol/L imidazole buffer. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

5 FIG 4 IgE immunoblotting of rCor a 8: lanes 1 to 26, patient sera 1 to 26; lane 27, nonallergic control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

6 FIG 5 Immunoblot inhibition of immobilized nCor a 8 (20 μg of hazelnut extract per centimeter) with serum pool: lane 1, buffer control; lane 2, nonallergic serum; lane 3, no inhibitor; lane 4, 15 mg of rCor a 8; lane 5, 700 μg of hazelnut extract. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

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