1. Cell composition of the human pulmonary valve: a comparative study with the aortic valve–the VESALIO∗ project∗ 
Foscarina Della Rocca, MD, Saverio Sartore, PhD, Diego Guidolin, PhD, Barbara Bertiplaglia, BSc, Gino Gerosa, MD, Dino Casarotto, MD, Paolo Pauletto, MD 
The Annals of Thoracic Surgery 
Volume 70, Issue 5, Pages (November 2000)
DOI: /S (00)

2. Fig 1
Immunocytochemistry on cryosections from pulmonary valve leaflets (A, C, E, G) and aortic valve leaflets (B, D, F, H) reacted with anti-SM α-actin (A, B), anti-SM22 (C, D), anticalponin (E, F), and anti-SM(-MyHC) myosin (G, H), monoclonal antibodies. Note the continuous subendocardial layer of positive cells in A (green arrows) and clusters of positive cells in C, E, G, and H (red arrows) in the outermost part of the fibrosa. In aortic valve leaflets, the layer of positive subendocardial cells is generally thicker than that of pulmonary valve leaflets (× 400).
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

3. Fig 2
Bar graph showing the distribution of positive cells (number of cells/μm2) with (A) antivimentin, (B) NM-F6 anti-MyHC-Apla1, and (C) NM-G2 anti-MyHC-Apla2 in fibrosa, spongiosa, and ventricularis from aortic valve leaflets (white bars) and pulmonary valve leaflets (black bars). Mean ± SD is reported.
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

4. Fig 3
Western blotting analysis of crude extracts from (1) aortic valve leaflets and (2) pulmonary valve leaflets electrophoresed in (A) 12.5% or (B) 5% SDS-gels, reacted with antivimentin, anti-SM22, and anti-SM (α)-actin is shown in panel A. In panel B, the pattern obtained with anti-SM(-MyHC), anti-MyHC-Apla1, and anti-MyHC-Apla2 antibodies is shown. The band reactive with the anti-NM myosin antibodies is about 200-kDa apparent mass. Note that SM22 and SM-MyHC antigens are undetectable under the conditions used in this experiment; a, b, and c indicated the Western blots.
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

5. Fig 4
Schematic representation of the various cell populations present in the fibrosa, spongiosa, and ventricularis of aortic valve leaflets (AVL) and pulmonary valve leaflets (PVL) as determined by immunocytochemical experiments. Fetal-type SM cells and myofibroblasts are segregated in the outermost layer of fibrosa, whereas different fibroblast subtypes are expressed in the ventricularis of AVL versus PVL and in the spongiosa of both valves as well. The slight difference existing in vimentin distribution between AVL and PVL at the level of fibrosa is not reported in the figure. The up arrows and down arrows indicate a high or low number of vimentin-positive cells, respectively.
The Annals of Thoracic Surgery  , DOI: ( /S (00) )