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The expression of inducible nitric oxide synthase in the human fallopian tube during the menstrual cycle and in ectopic pregnancy  Majedah Al-Azemi, F.R.C.O.G.,

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Presentation on theme: "The expression of inducible nitric oxide synthase in the human fallopian tube during the menstrual cycle and in ectopic pregnancy  Majedah Al-Azemi, F.R.C.O.G.,"— Presentation transcript:

1 The expression of inducible nitric oxide synthase in the human fallopian tube during the menstrual cycle and in ectopic pregnancy  Majedah Al-Azemi, F.R.C.O.G., Bassem Refaat, Ph.D., Saad Amer, M.D., Bolarinde Ola, M.D., Neil Chapman, Ph.D., William Ledger, D.Phil.  Fertility and Sterility  Volume 94, Issue 3, Pages (August 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Immunohistochemical localization of iNOS enzyme in the ampullary (C, E, G, I, and K) and isthmic (D, F, H, J, and L) regions of human fallopian tube sections collected from ectopic pregnancy (C and D) and pseudopregnant groups (E and F) and during the different stage of the menstrual cycle (G–L). Human endometrium was used as a positive control tissue for iNOS enzyme (A). No immunoreactivity was observed in negative control using blocking peptide for iNOS antibody (B). (A–C, E, F, I, and J taken at ×400 magnification; G and H taken at ×250 magnification; and D taken at ×100 magnification. A, B, G, and H, scale bar = 5 μm; I and J, scale bar =10 μm; C, E, and F, scale bar = 7 μm; D, scale bar = 15 μm.) Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Quantitative RT-PCR analysis of β-actin and iNOS enzyme in fallopian tubes during the different stages of the menstrual cycle, ectopic pregnancy and pseudopregnant groups. (A) Quantitative RT-PCR analysis of β-actin expression in the fallopian tubes collected during the different stages of the menstrual cycle. P > (B) Quantitative RT-PCR analysis of the iNOS in the fallopian tubes collected during the different stages of the menstrual cycle. (C) Quantitative RT-PCR analysis of β-actin in the ampullary region (AMP) and isthmic region (ISTH) of fallopian tubes collected from ectopic pregnancy (EP) and pseudopregnant (SP) groups. (D) Quantitative RT-PCR analysis of the iNOS enzyme in the AMP and ISTH of fallopian tubes collected from EP and SP groups. ∗P < 0.05. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions


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