1. Distribution of disorder in the cytosolic phosphoproteome
Distribution of disorder in the cytosolic phosphoproteome.A, the distribution of sequence disorder (percentage per protein) versus the longest disordered region (per phosphoprotein) was investigated using the PONDR VL-XT Predictor.
Distribution of disorder in the cytosolic phosphoproteome.A, the distribution of sequence disorder (percentage per protein) versus the longest disordered region (per phosphoprotein) was investigated using the PONDR VL-XT Predictor. Three categories of disorder were established: those that are highly disordered (>70% sequence disorder, 40 phosphoproteins), those that are mostly disordered (50–70% sequence disorder, 45 phosphoproteins), and those that are partly disordered (<50% sequence disorder, 56 phosphoproteins). 133 of 141 cytosolic phosphoproteins contain a disordered region of >40 residues, a length that is generally considered a long disordered region and can be predicted with 98.3% accuracy using PONDR. B, comparison of the number of phosphorylation sites (normalized to the number of disordered residues) with the length of disordered regions is shown. The majority of sites were located in a long region of disorder (>40 residues), and over 200 phosphorylation sites were in regions of disorder that were >100 residues long. The increased relative density of phosphorylation sites in very long disordered regions represents the highly phosphorylated and disordered proteins in the data set such as RNA-binding and spicing proteins.
Mark O. Collins et al. Mol Cell Proteomics 2008;7:
© 2008 The American Society for Biochemistry and Molecular Biology