1. Effect of gonadotropins on dynamic events and global deoxyribonucleic acid methylation during in vitro maturation of oocytes: an animal model 
Shan Liu, M.D., Ph.D., Huai L. Feng, Ph.D., Dennis Marchesi, M.Sc., Zi-Jiang Chen, M.D., Ph.D., Avner Hershlag, M.D. 
Fertility and Sterility 
Volume 95, Issue 4, Pages e3 (March 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Effects of different Gn concentrations or combinations on spindle configuration and chromosomal alignment. (A1) Normal spindle and chromosomes in bovine oocytes; (A2, A3) slightly abnormal and abnormal configurations of spindle and chromosomes, respectively. Image in upper left of (A2) shows a magnified spindle, in which distortion of the microtubules can be seen (arrowhead). Green, β-tubulin staining; red, DNA staining; yellow, merge of green and red fluorescence. Bar = 50 μm. (B–D) Effects of different concentrations of, respectively, B, B+M, and B+R supplementation during IVM on spindle configuration and chromosomal alignment. ∗P<.05 vs. control.
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3. Effects of different Gn concentrations or combinations on mitochondrial distribution. (A1) Mitochondria distributed homogeneously, most located in the peripheral region; (A2) granular mitochondria; (A3) mitochondria distributed in clusters. Bar = 50 μm. (B–D) Distribution of mitochondria in bovine oocytes matured with varying doses of B, B+M, and B+R, respectively.
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4. Effects of different Gn concentrations or combinations on CG distribution. (A1) Cortical granule distributed in clusters throughout the cytoplasm; (A2) CG partly dispersed; (A3) CG dispersed. Bar = 50 μm. (B–D) Cortical granule distribution in bovine oocytes matured in different concentrations of B, B+M, and B+R, respectively. ∗P<.05 vs. control.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Effects of different Gn concentrations or combinations on global DNA methylation status. (A) Typical staining of 5-MeC in bovine metaphase II oocytes. Green, 5-MeC staining; red, DNA staining. Bar = 50 μm. (B) Levels of global DNA methylation in oocytes matured in different concentrations of F, F+M, and F+R.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions