1. Mineralization of articular cartilage in the sprague-dawley rat: characterization and mechanical analysis 
M.L. Roemhildt, B.D. Beynnon, M. Gardner-Morse 
Osteoarthritis and Cartilage 
Volume 20, Issue 7, Pages (July 2012)
DOI: /j.joca
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
A) Superior view of rat tibial plateau showing isosurface of reconstructed μCT image and areas of mineralization within the articular cartilage (◂), (B) Photograph of the same specimen in which areas of well-developed mineralizations appear as white spots on the articular surface (◂), (C) Representative backscattered electron image of a coronal section of the medial compartment of the tibia plateau illustrating the articular surface (ǂ), mineralized region of articular cartilage (→) and underlying subchondral bone, (D) Magnified image of mineralized region depicted in Panel C illustrating the mineralized area (*) and underlying calcified cartilage (#). Numbered points illustrate some of the points at which energy dispersive spectra were collected, (E) Representative energy dispersive spectra of two mineralized particles illustrating the main components of the mineralized regions are phosphorus (P), calcium (Ca) and oxygen (O) with minor traces of magnesium (Mg) and sodium (Na).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Coronal osteochondral sections showing representative cyst-like regions (◂) in the articular cartilage, which were mineralized prior to histological processing, stained with Hematoxylin and Eosin (A–D) and corresponding serial section below stained with SOFG (E–H). The substructure within these regions represent vestiges where large mineral deposits resided and coincide with mineralized regions as observed by μCT and surface white spots (Fig. 1 A-B). Little Safranin O staining was observed within cysts.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions