1. Volume 148, Issue 2, Pages 295-297 (February 2015)
Quantitative Assessment of Liver Steatosis on Tissue Section Using Infrared Spectroscopy 
François Le Naour, Laurent Gadea, Mélanie Danulot, Ibraheem Yousef, Eric Vibert, Mathieu Wavelet, Slávka Kaščáková, Denis Castaing, Didier Samuel, Paul Dumas, Catherine Guettier 
Gastroenterology 
Volume 148, Issue 2, Pages (February 2015)
DOI: /j.gastro
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2. Figure 1
(A) Tissue sections from paraffin embedded or frozen surgical samples were stained with H&E. (B) Steatosis estimated on frozen tissue section after H&E staining was plotted as a function of triglycerides (TG) concentration measured from the same surgical specimen by GC-MS (r2 = 0.54). (C) Infrared microspectroscopy analysis was performed on a huge area (white dashed line) of unstained frozen tissue section that was rastered by IR beam with aperture size of 50 x 50 μm2. On each IR spectrum, the relative amount of lipids and proteins was estimated by calculating the area of the bands 3100–2800 cm-1 and 1595–1485 cm-1. (D) The average ratio lipids/proteins was calculated for each studied patient and plotted as a function of TG concentration measured from the same surgical specimen (r2 = 0.92). (E) A set of surgical specimen obtained at harvesting of liver grafts right before clamping was investigated by IR microspectroscopy and GC-MS.
Gastroenterology  , DOI: ( /j.gastro )
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3. Figure 2
(A) ATR-FTIR spectrometer connected to a computer (upper panel). A tissue section is deposited on a glass slide used for histology. The glass slide is placed on the spectrometer and the sample maintained in close contact with the detector (lower panel). (B) Results of the analysis on the computer screen.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions