1. Ogami_Fig. S1 CPEB pHA-CMV5- 1.5 3 Time (h) oligo(dT)/ RNase H
1.5 3
Time (h)
oligo(dT)/ RNase H
GST-CAT mRNA
BGG
c-myc 3’UTR
mRNA 1 2 3 4 5 6 7 8 9 10

2. A B C Ogami_Fig. S2 MS2-HA -CPEB ( ) MS2-HA ( ) HA-CPEB ( ) (kb) 2 4 6
( )
HA-CPEB ( )
(kb) 2 4 6 8 2 4 6 8 2 4 6 8
Time (h)
1.5
EGFP mRNA
1.0
0.8
BGG(1-39) MS2bs mRNA
0.6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 B C
100
MS2-HA-CPEB
MS2-HA
HA-CPEB
(kDa)
RNA level (%) 89
MS2-HA-CPEB 10 63
HA-CPEB
MS2-HA
(WB: anti-HA)
MS2-HA-CPEB
GAPDH
HA-CPEB 35
(WB: anti-GAPDH) 1 2 4 6 8
Time (h)

3. Ogami_Fig. S3 pCMV-5XMyc-PARN CPEB CPEB3 CPEB CPEB3 pCMV-5XFlag-
anti-Flag
anti-Flag
anti-Flag
(kDa) IP 93
5XMyc-PARN
(WB: anti-Myc)
5XFlag-CPEB3 93
5XFlag-CPEB 62
(WB: anti-Flag) 1 2 3 4 5 6

4. Ogami_Fig. S4 A B
mature
(a-c)
pre
(b-c)
Maker RT