1. iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia
Lactose Intolerance
iGEM Meeting #7
08/06/08
Presentation by Robert Ovadia

2. iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia
System Design
The idea behind using RBS’s is we want to use the weakest RBS for lacY so we can pump the strongest promoter. We want to express LacZ as much in order to do this.
*ADD ORI’s*
iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

3. iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia
System Design
By next week, you guys should be telling me how the plasmids look like. I will test you!
*ORI’s*
iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

4. Two Weeks Left The Final Push: What We Want33
LacY 34
LacZ
SDM +2H
We have: 33
LacY 34
LacZ
SDM +H
SDM +2H??
iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

5. Two Weeks Left The Final Push: What We Want
Lysis Cassette
A81 / B4
In pSB2K3
We have:
Lysis Cassette
In pSB2K3
iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia